沙棘与疏肝理气及清热利湿方配伍对脂肪肝大鼠的防治作用  

Preventative and curative function of Hippophae rhamnoides L combined with Shugan Liqi or Qingre Lishi drugs in mice with fatty liver diseases

作　　者：吴振宇[1] 张云[1] 李永海[2] 吴广均[1] 张红梅 温中平 刘晓梅[1] 王荣梅[1] 

机构地区：[1]中国中医科学院广安门医院免疫研究室,北京市100053 [2]水利部沙棘开发管理中心,北京市100038 [3]高原圣果沙棘制品有限公司,北京市100038

出　　处：《世界华人消化杂志》2008年第7期701-705,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨沙棘分别与逍遥散及二妙散配伍对实验性脂肪肝大鼠的防治作用.方法:大鼠随机分为5组:正常组(N)、模型组(M)、西药(东宝肝泰)组(D)、沙棘配逍遥丸组(SA)、沙棘配二妙散组(SB),每组10只.正常组基础饲料喂养,其余各组高脂饲料喂养8wk后行药物干预,并继续高脂每日加250mL/L乙醇12mL/kg,ig,1mo后观察进食量、体质量、肝脾指数、用药前后血清总胆固醇(CHO,mmol/L)、三酰甘油(TG,mmol/L)、低密度脂蛋白(LDL,mmol/L)、极低密度脂蛋白(VLDL,mmol/L),高密度脂蛋白(HDL,mmol/L)及肝脏病理改变.结果:高脂喂养2mo和药物干预后,与N组相比,M组大鼠体质量增长加快,CHO,TG,LDL和VLD明显升高,HDL明显下降;与M组相比,SA和SB组大鼠进食量减少、TG和VLDL下降(TG:3.9±0.2,4.0±0.1vs5.4±0.3;VLDL:2.4±0.1,2.7±0.1vs3.1±0.2,P<0.05),D组TG下降(3.3±0.5vs5.4±0.3,P<0.05).肝脏病理方面,M组肝细胞呈广泛的气球样变,SA和SB组均能明显减少病变的肝细胞数和减轻病变程度.结论:沙棘与逍遥散及二妙散配伍对肝脏损伤有保护作用,以沙棘与逍遥散配伍防治作用更好。AIM： To explore the role of Hippophae rhamnoides L in combination with Shugan Liqi drugs （Xiao Yao San） or Qingre Lishi drugs （Er Miao San） respectively in experimental mice with fatty liver diseases. METHODS： Rats were randomly divided into five groups： normal group （N）, model （M）, Dongbao Gantai （D）, Hippophae rhamnoides L with Xiao Yao San （SA） or Er Miao San group （SB）; in each group there were 10 rats. The rats in group N were fed with basic feed; the rats in the other groups were fed with food containing high fat for 8 wk, and then treated with the corresponding drugs while fed with high-fat diet and 250 mL/L alcohol 12 mL/kg per day for 1 mo. We observed the quantity of food intake, body weight, index of liver and spleen, and serum levels （mmol/L） of cholesterol （CHO）, triglycerides （TG）, low density lipoprotein （LDL）, very low density lipoproten （VLDL） and high density lipoproten （HDL） as well as liver pathology before and after drug administration. RESULTS： Two months after high-fat diet feeding and intervention of drugs, compared with those in group N, the body weights were increased in group M; the levels of serum CHO, TG, and VLD were obviously increased, and those of LDL and HDL were decreased dramatically. In comparison with those in group M, TG and VLDL levels were decreased in group SA and SB （TG： 3.9 ± 0.2, 4.0 ± 0.1 vs 5.4 ± 0.3; VLDL： 2.4 ± 0.1, 2.7 ± 0.1 vs 3.1 ± 0.2, P 〈 0.05）, and the quantity of food intake was also reduced; meanwhile, TG level was decreased in group D （3.3 ± 0.5 vs 5.4 ± 0.3, P 〈 0.05）. In group M, balloon-like liver cells were extensively observed, while in groups SA and SB, the number and denaturalized severity of rat liver cells reduced. CONCLUSION： Hippophae rhamnoides L combined with Xiao Yao San or Er Miao San can protect liver against the damages caused by alcohol and high fat, and the combination of Hippophae rhamnoides L with Xiao Yao San has a better preventative function.

关 键 词：沙棘 高脂饲料 酒精 脂肪肝 

分 类 号：R285[医药卫生—中药学]