3种海参的皂苷对血管内皮细胞作用的比较研究  

Comparative Investigation on the Effects of Triterpene Glycosides from Three Kinds of Sea Cucumber on Vascular Endothelial Cells

作　　者：王静凤[1] 高森[1] 逄龙[1] 赵芹[1] 刘鹤[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2008年第2期221-224,280,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30471319);国家高技术研究发展计划项目(2007AA091805);中国海洋大学海水养殖教育部重点实验开放课题资助

摘　　要：比较研究了日本刺参(Apostichopus japonicus,A.japonicus)、北极刺参(Thelenata anax,T.anax)和菲律宾刺参(Pearsonothuria graeffei,P.graeffei)的皂苷对氧化损伤血管内皮细胞的作用。实验结果显示,对于正常血管内皮细胞,日本刺参皂苷无细胞毒作用,而北极刺参和菲律宾刺参皂苷具有显著的细胞毒作用。对于氧化型低密度脂蛋白(ox-LDL)氧化损伤的血管内皮细胞,日本刺参皂苷能显著提高细胞增殖活性(P<0.01),抑制其凋亡(P<0.01),而北极刺参和菲律宾刺参皂苷无显著影响。另外,3种海参的皂苷均能显著降低细胞内丙二醛(MDA)含量(P<0.01),但对细胞NOS活性和NO释放量无显著影响。提示日本刺参皂苷能够有效保护氧化损伤血管内皮细胞,对动脉粥样硬化(Atherosclerosis,AS)的防治具有重要意义。而北极刺参和菲律宾刺参的皂苷对血管内皮细胞无保护作用。Comparative investigation of the effects of triterpene glycosides（TrG） from Apostichopus japonicus （ A . japonicus ）, Thelenata anax （ T. anax ） and Pearsonothuria graeffei （ P . graeffei ） on vascular endothelial cells was conducted. The results indicated that there is no cytotoxic effect on the normal cells of TrG from A. japonicus, whereas the TrG from T. anax and P. graeffei has significant cytotoxic effect. As to vascular endothelial cells injured by ox-LDL, the TrG from A. japonicus could stimulate cell proliferation activity（P 〈 0.01） and inhibit cell apoptosis（P 〈 0.01） significantly; however, TrG from T. anax and P. graeffei showed no effect. In addition, TrG from these three species of sea cucumber decreased the content of MDA（P 〈 0.01） in injured ECV304 cells, but no significant effect either on the activity of NOS or the releasing of NO was detected. It can be concluded that TrG from A. japonicus can protect ECV304 from oxidative injuries effectively which in turn has affirmative significance in the prevention and treatment of Atherosclerosis（AS）. However, TrG from T. anax and P. graeffei showed no potent effect.

关 键 词：日本刺参 北极剌参 菲律宾剌参 皂苷 血管内皮细胞 

分 类 号：R282.74[医药卫生—中药学] R331.36[医药卫生—中医学]