不同途径接种乙脑病毒prME蛋白编码基因DNA疫苗所致BALB/c鼠的细胞免疫  

Cellular immunity of BALB/c mice induced by DNA vaccine encoding prME protein derived from Japanese encephalitis virus in the routes of different inoculation

作　　者：周言[1] 张琳[1] 翟永贞[1] 李喜梅[1] 王占英[1] 冯国和[1] 

机构地区：[1]中国医科大学附属盛京医院感染科,辽宁沈阳110004

出　　处：《中国医科大学学报》2007年第6期625-627,共3页Journal of China Medical University

基　　金：国家自然科学基金(30471547);教育部留学回国人员启动基金(教外司留[2004]);辽宁省自然科学基金(20042072);辽宁省教育厅重大基础研究计划基金(05L498)资助项目

摘　　要：目的:研究经不同途径接种乙脑病毒prME蛋白编码基因DNA疫苗所诱导的细胞免疫。方法:将乙脑病毒prME蛋白编码基因DNA疫苗(命名为pJME)分别以肌注、滴鼻两种免疫方式免疫BALB/c鼠,流式细胞仪检测脾脏CD4+/CD8+T淋巴细胞亚群,乳酸脱氢酶释放实验测定脾脏特异性细胞毒性T淋巴细胞(CTL)活性。结果:pJME肌注组与滴鼻组免疫鼠CD4+T淋巴细胞百分比(30.91+0.72)%、(27.89+0.42)%,均高于灭活疫苗与载体肌注组(23.73+0.17)%、(23.78+0.21)%(P<0.05),肌注组显著高于滴鼻组(P<0.05);而CD8+T淋巴细胞百分比(14.62+0.25)%、(13.73+0.50)%,也均高于灭活疫苗和载体肌注组(10.80+0.39)%、(11.97+0.06)%(P<0.05),肌注组与滴鼻组无显著差异(P>0.05);两组有明显CTL效应(pJME肌注组29.1%,pJME滴鼻组17.1%),明显高于载体肌注组6.0%,pJME肌注组明显优于滴鼻组。结论:pJME以两种途径免疫鼠时可诱导明显细胞免疫反应。Objective：To study cellular immunity induced by DNA vaccine encoding prME protein derived from Japanese encephalitis virus in the routes of different inoculation.Methods：BALB/c mice were vaccinated with DNA vaccine,named pJME,encoding Japanese Encephalitis virus prME protein via intramuscular and intranasal injection respectively.CD4＋/CD8＋ T lymphocyte subsets of mouse spleen cells were assayed with flow cytometry technique,and CTL activity was detected with lactic dehydrogenase release test method.Results：Both of CD4＋ T lymphocyte percentage [intramuscular：（30.91＋0.72）%,intranasal：（27.89＋0.42）%] produced by pJME via intramuscular and intranasal injection respectively were higher than that by inactivated vaccine and vector.[inactivated vaccine：（23.73＋0.17）%,vector：（23.78＋0.21）%]（P 〈 0.05）,however,pJME group via intramuscular injection was still higher than that via intranasal injection（P 〈 0.05）.Both of CD8＋ T lymphocyte percentage [intramuscular：（14.62＋0.25）%,intranasal：（13.73＋0.50）%]produced pJME via intramuscular and intranasal injection respectively were also higher than that by inactivated vaccine and vector[inactivated vaccine：（10.80＋0.39）%,vector：（11.97＋0.06）%]（P 〈 0.05）,but there was no significant difference（P 〉 0.05） between pJME group via intramuscular injection and that via intranasal injection.Both of above groups could promote CTL activity（pJME intramuscular：29.1%,pJME intranasal：17.1%）.pJME group via intramuscular injection was higher than that via intranasal injection.Conclusion：pJME via intramuscular and intranasal injection respectively can induce obvious cellular immunity.

关 键 词：脑炎 日本 疫苗 DNA T淋巴细胞亚群 T淋巴细胞 细胞毒性 

分 类 号：R512.32[医药卫生—内科学]