瑞芬太尼、舒芬太尼和芬太尼对蟾蜍上腭纤毛摆动频率的影响  

Effect of remifentanil,sufentanil and fentanyl on cilia beat frequency of isolated toad palate

作　　者：印春铭[1] 何芳[1] 于洋[1] 王晓芳[1] 

机构地区：[1]中国医科大学附属第四医院麻醉科,辽宁沈阳110032

出　　处：《中国医科大学学报》2007年第6期656-657,共2页Journal of China Medical University

基　　金：辽宁省教育厅高等学校科研基金资助项目(05L562);沈阳市科学技术计划技术创新开发研究计划资助项目(1053125-1-54)

摘　　要：目的:对比瑞芬太尼、舒芬太尼和芬太尼对蟾蜍上腭纤毛摆动频率(CBF)的影响。方法:取30只中华大蟾蜍,每只在显微镜下从上腭黏膜分离出一个约2mm×2mm的黏膜块,随机分为瑞芬太尼组、舒芬太尼组和芬太尼组。生理盐水稀释瑞芬太尼、舒芬太尼和芬太尼,终浓度分别为4,0.5,2μg/L,每个黏膜块在浸入药液前及浸入后5,30和60min时,使用相差显微镜及成像分析技术测量纤毛上皮的CBF。结果:舒芬太尼组和芬太尼组CBF在各时间点与基础值比较均无显著差异(P>0.05);瑞芬太尼组CBF在各时间点与基础值比较明显降低(P<0.05)。各组CBF基础值无显著差异(P>0.05);在其他时间点,瑞芬太尼组CBF较舒芬太尼和芬太尼组明显降低(P<0.05)。结论:瑞芬太尼降低蟾蜍上腭纤毛摆动频率,舒芬太尼和芬太尼则没有影响。Objective：To investigate and compare the effects of remifentanil,sufentanil and fentanyl on cilia beat frequency（CBF） of isolated toad palate.Methods：The palates were sampled from 30 matured toads.The mucosa of palates was cut into 2 mm × 2 mm small pieces under the stereoscope.With normal saline remifentanil,sufentanil and fentanyl were diluted to 4μg/L、0.5μg/L and 2μg/L respectively.The small pieces were randomly divided into three groups to dip into remifentanil,sufentanil or fentanyl.Actively beating ciliated cells were observed and their motions were quantified by measuring CBF using phase-contrast microscopy.CBF was counted manually at baseline,5,30 and 60 min respectively.Results：CBF in remifentanil decreased significantly at the points of 5,30 and 60 min compared with that at the baseline（P 〈 0.05）,but CBF in sufentanil group and fentanyl group were not changed obviously（P 〉 0.05）.CBF of all the groups had no difference at the baseline（P 〉 0.05）.Compared with sufentanil and fentanyl group,CBF in remifentanil decreased significantly at the points of 5,30 and 60 min（P 〈 0.05）.Conclusion：Remifentanil decreases CBF of isolated toad palate.

关 键 词：瑞芬太尼 舒芬太尼 芬太尼 纤毛摆动频率 

分 类 号：R614.2[医药卫生—麻醉学]