金黄色葡萄球菌α毒素对肺水通道蛋白表达的影响  

Effect of S.Aureus α-toxin on pulmonary aquaporin-1 expression in mice

作　　者：李玲[1,2] 李尔然[1] 夏淑杰[3] 林庶茹[3] 李艳杰[1] 才丽萍[3] 王秋月[1] 康健[1] 

机构地区：[1]中国医科大学附属第一医院呼吸疾病研究所,辽宁沈阳110001 [2]沈阳市胸科医院三病房 [3]辽宁中医学院基础医学院分子生物学实验室

出　　处：《中国医科大学学报》2007年第6期692-693,727,共3页Journal of China Medical University

基　　金：国家自然科学基金(30470766);高等学校博士学科点专项科研基金(20040159011);教育部留学归国人员科研启动基金(2004527)资助项目

摘　　要：目的:观察金黄色葡萄球菌α毒素(α-toxin)对Balb/c小鼠肺微血管内皮细胞(LMEC)水通道蛋白1(AQP1)表达的影响。方法:将实验组小鼠15只腹腔注入α-toxin后根据6h、10h、24h取材时间,随机将它们分为α-toxin6h组、α-toxin10h组和α-toxin24h组。取左肺行病理HE染色和免疫组化染色,检测肺组织病理变化情况和AQP1表达的变化。结果:α-toxin各组肺泡间隔增宽、水肿,炎性细胞浸润,肺泡结构破坏,以α-toxin10h组最为明显。免疫组化α-toxin各组AQP1表达下降(P<0.05)。其中以α-toxin10h组最为明显。α-toxin6h组和α-toxin10h组及α-toxin10h组和α-toxin24h组均有显著性差异(P<0.05)。结论:AQP1表达于LMEC。金葡菌α毒素可致小鼠肺LMEC的AQP1表达下降,随着炎症的减轻,AQP1也随之表达增加,进而恢复正常。Objective：To observe the effect of S.aureus alpha toxin on pulmonary aquaporin-1（AQP1） expression in mice.Methods：Balb/c mice were divided into alpha toxin-6 h group,alpha toxin-10 h group and alpha toxin-24 h group according to the sampling time after injecting alpha toxin peritoneally.Immunohistochemistry stain for pulmonary AQP1 was done after execution.Results：Edema and inflammatory cell infiltration were shown in alveolar wall in all alpha toxin groups,especially obviously in alpha toxin-10 h group.AQP1 expression decreased in these groups,of which alpha toxin-10 h group was most obvious.Conclusion：AQP1 is mainly localized on lung microvascular endothelial cells.AQP1 expression decreased after mice are exposed to S.aureus alpha toxin,while down-regulated AQP1 return to normal level overtime.

关 键 词：Α毒素 水通道蛋白1 肺微血管内皮细胞 

分 类 号：R563.8[医药卫生—呼吸系统]