正常人和肿瘤患者CIK细胞的体外增殖能力及抗肿瘤作用的对比分析  

Analysis on CIK cell's proliferation ability and antineoplastic activity in vitro between healthy adults and tumor patients

作　　者：梁红[1] 王晓华[1] 隋承光[1] 孟凡东[1] 傅立业[1] 姜又红[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第二研究室,辽宁沈阳110001

出　　处：《中国医科大学学报》2007年第6期706-708,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20042093)

摘　　要：目的:探讨正常人和肿瘤患者CIK细胞体外增殖能力及抗肿瘤作用的差异。方法:分离正常人和肿瘤患者的外周血单个核细胞(PBMC),加入细胞因子(CK),体外诱导CIK细胞,用细胞计数板法观察CIK细胞增殖能力及随时间变化的趋势,用MTT法检测其对不同肿瘤细胞株的杀伤活性。结果:与正常人CIK细胞相比,肿瘤患者CIK细胞的增殖速度较慢,其最大增殖倍数低(P<0.01);CIK细胞的增殖速度与年龄有关,即低年龄段的CIK细胞增殖能力强;正常人的CIK细胞对乳腺癌细胞株(MDA-MB-231)、肺巨细胞癌细胞株(BE-1)和前列腺癌细胞株(PC-3)的杀伤活性明显高于肿瘤患者的CIK细胞(P<0.01);不同年龄段肿瘤患者的CIK细胞对肿瘤细胞的杀伤率差异有统计学意义(P<0.05)。结论:CIK细胞的体外增殖力及对肿瘤细胞的杀伤活性与年龄有关,正常人CIK细胞的体外增殖力及对肿瘤细胞的杀伤活性高于肿瘤患者的CIK细胞。Objective：To investigate the differences in CIK’ proliferation ability and antineoplastic effect in vitro between healthy adults and tumor patients.Methods：The CIK amplification ability and time-dependent tendency with culture continuing was tested by blood cell recording board;The CIK cytotoxicity was tested by MTT assays.Results：Compared with normal adults,tumor patients’ CIK proliferated more slowly and its max proliferation multiple is lower（P 〈 0.01）.Meanwhile,the proliferation ability was related to age（P 〈 0.01）.MTT assays showed that the cytotoxicity of normal adults’ CIK against tumor cells（MDA-MB-231,BE-1,PC-3）was significantly stronger than that of tumor patients;The disparity in cytoxic rate of the tumor patients’ CIK against tumor cells from different age groups was statistically significant（P 〈 0.05）.Conclusion：CIK amplification ability and cytotoxic activity in vitro are related to age;The proliferation ability and killing activity of normal human CIK in vitro is stronger than that of tumor patients.

关 键 词：CIK细胞 外周血单个核细胞 细胞因子 体外增殖 抗肿瘤作用 

分 类 号：R392.12[医药卫生—免疫学]