龟鹿二仙胶抵抗化疗小鼠脾T淋巴细胞凋亡的实验研究  

Effect of Guilu Erxianjiao in Suppressing Splenic T-Lymphocyte Apoptosis in Mice Undergoing Chemotherapy

作　　者：林胜友[1] 沈敏鹤[1] 陈健[1] 徐莉[2] 刘振东[1] 蒋福升[2] 丁志山[2] 

机构地区：[1]浙江省中医院,杭州310006 [2]浙江中医药大学生命科学系

出　　处：《中国中西医结合杂志》2008年第4期339-342,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省自然基金科研项目(No.X205007)

摘　　要：目的探讨龟鹿二仙胶对化疗小鼠的抗凋亡作用及作用机理。方法将荷瘤昆明种小鼠72只随机分为环磷酰胺(CTX)加龟鹿二仙胶高、中、低3个剂量组,CTX组,单纯中药组,生理盐水组6组。给药9天后处死小鼠取脾组织,采用流式细胞仪检测T淋巴细胞凋亡;RT-PCR法检测bcl-2、Caspase-3 mRNA的表达。结果CTX组荧光素标记的膜联蛋白阳性(FITC+AnnexinV)/碘化丙啶(PI)染色阴性比例(FITC+/PI-)T细胞较生理盐水组增高,差异有统计学意义(P<0·05);CTX加用龟鹿二仙胶的3个剂量组FITC+/PI-T细胞比例较CTX组明显降低(P<0·05)。CTX组bcl-2 mRNA表达量较生理盐水组减少(P<0·05);CTX加用龟鹿二仙胶的3个剂量组其表达量较CTX组明显升高(P<0·05)。Caspase-3 mRNA表达量CTX组升高(P<0·05);CTX加用龟鹿二仙胶的3个剂量组表达量较CTX组明显减少。结论龟鹿二仙胶能有效抑制化疗小鼠淋巴细胞凋亡。上调bcl-2 mRNA表达、下调Caspase-3 mRNA表达可能是其作用机理之一。Objective To investigate the effect of Guilu Erxianjiao （GLEXJ） in suppressing chemotherapy induced cell apoptosis in mice and to discuss its possible acting mechanism. Methods Seventy-two tumor-bearing Kunming mice were randomly divided into 6 groups, Group A, treated with normal saline; Group B treated with cyclophosphamide （CTX）, Group C treated with Chinese herbal medicine, and Group D-F, the three combined treated group received CTX plus high, moderate and low dose GLEXJ. All mice were sacrificed after 9-day medication, their splenic tissues were taken for determining T-lymphocyte apoptosis with flow cytometer, the expression of bcl-2 mRNA and Caspase-3mRNA detected by RT-PCR. Results As compared with Group A, FITC^＋/PI^- cell proportion of spleen T-lymphocyte cells and Caspase-3mRNA expression were higher, while bcl-2 mRNA expression was lower in Group B; as compared with Group B, the former two indexes were lower and the latter was higher in the three combined treated groups （all P 〈 0.05）. Conclusion GLEXJ could efficiently suppress the splenic T-lymphocyte apoptosis in tumor bearing mice undergoing chemotherapy, one of its mechanisms may be through up-regulating of bcl-2 mRNA expression and down-regulating of Caspase-3mRNA expression.

关 键 词：龟鹿二仙胶 凋亡 BCL-2 MRNA CASPASE-3MRNA 

分 类 号：R285.5[医药卫生—中药学]