补阳还五汤对脑出血大鼠脑内促血管生成素-1及其受体mRNA表达的影响  

Effect of Buyang Huanwu Decoction on Expressions of Angiopoietin-1 and Its Receptor mRNA in Brain of Rat after Intracerebral Hemorrhage

作　　者：周华军[1] 唐涛[1] 钟建华[1] 罗杰坤[1] 刘小娟[1] 虢灿杰[1] 杨期东[2] 张海男[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙410008 [2]中南大学湘雅医院神经病学研究所

出　　处：《中国中西医结合杂志》2008年第4期343-347,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学青年基金(No.30400581);中国博士后科学基金(No.2005038224);湖南省重点学科建设经费(No.湘教通〔2001〕179号)资助

摘　　要：目的通过观察补阳还五汤对脑出血大鼠脑内损伤区促血管生成素-1（angiopoietin-1，Ang-1）及其内皮特异性受体（endothelial-specific receptor tyrosine kinase，Tie-2）mRNA表达的影响，探讨补阳还五汤的作用机制。方法160只SD大鼠随机分为正常组（10只）、假手术组（60只）、模型组（60只）、补阳还五汤（BYHWD）组（30只），通过立体定位向脑内苍白球注入Ⅶ型胶原酶0．5U建立脑出血模型。其中正常组自由饮水，假手术组和模型组灌服蒸馏水，BYHWD组灌服补阳还五汤。采用免疫组化方法检测假手术组和模型组1、4、7、14、21、28天各时间点Ang-1及其受体表达位置变化。运用逆转录聚合酶链式反应（reverse transcription-polymerase chain reaction，RT-PCR）检测正常组及其它各组造模术后1、4、7、14、21、28天各时间点Ang-1和Tie-2mRNA的动态变化。结果正常组和假手术组不同时间点Ang-1和Tie-2未见明显表达；模型组1-4天即有Ang-1／Tie-2阳性微血管段在血肿边缘表达，且从7天开始阳性微血管段逐渐伸入血肿区。模型组在脑出血术后1天Ang-1和Tie-2mRNA即有表达，到4天其表达仍然很微弱，与1天比较无明显的差异，随后两者表达逐渐增多，到28天时达到高峰（P〈0．05）；补阳还五汤组在术后第7天开始两者的表达即显著高于模型组同-时间点水平（P〈0．05），且补阳还五汤组在术后21天即达到高峰（P〈0．01）。结论补阳还五汤能促进脑出血大鼠脑内Ang-1和Tie-2mRNA表达的上调，可能在脑出血后微血管系统重建过程中促进血管生成，从而促进损伤组织的修复。Objective To investigate the mechanisms of Buyang Huanwu Decoction （BYHWD） by observing its effects on expressions of angiopoietin-1 （Ang-1） and the endothelial-specific receptor tyrosine kinase （Tie-2） mRNA in damaged region of rats＇ brain after intracerebral hemorrhage （ICH）. Methods One hundred and sixty Sprague-Dawley rats were randomly divided into four groups, 10 in the normal control group, 60 in the sham-operative group, 60 in the ICH model group, and 30 in the BYHWD-treated group. The ICH model was established by injecting collagenase type VII 0, 5 U stereotaxically into fight globus pallidus, Animals in the BYHWD-treated group were administered orally with BYHWD, while animals in the sham-operative group and the ICH model group were administered orally with equal volume distilled water, and those in the normal control group drank water freely. The positional variations of the expression of Ang-1 and Tie-2 in the sham-operative group and the model group were assayed by immunohistochemistry on dayl, 4, 7, 14, 21 and 28 after modeling, in the meantime, the dynamic changes of Ang-1 and Tie-2 mRNA expressions in all groups were assayed by reverse transcription-polymerase chain reaction （RT-PCR）. Results No significant expression of Ang-1 and Tie-2 in brain of rats in the normal or the sham-operative group was found during the experiment. In the model group, the Ang-1 and Tie-2 positive micran-gio-segments appeared at the edge of clot on day I to day 4, they gradually penetrated to hematoma area from day 7 ; with Ang-1 and Tie-2 mRNA expressed from day 1, but very weak until day 4, showing no significant difference to that on day 1 ; thereafter, they increased gradually, and reached the peak on day 28 （P 〈 0. 05 ）. While the two expressions in the BYHWD treated group reached the peak on day 21, and from day 7 to day 28, they were all significantly higher than those in ICH model group at the corresponding time points （P 〈 0. 01 ）. Conclusion BYHWD can promote the up-regulation

关 键 词：脑出血 促血管生成素-1 受体 补阳还五汤 逆转录聚合酶链式反应 

分 类 号：R285[医药卫生—中药学]