高效液相色谱法测定烟叶中的总茄尼醇  

Determination of Total Solanesol in Tobacco Leaf with HPLC

作　　者：童康琼[1] 兰明蓉 赵云飞 冀志霞[1] 陈守文[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉市430070 [2]中国烟草白肋烟试验站,武汉市430030

出　　处：《烟草科技》2008年第3期49-52,共4页Tobacco Science & Technology

摘　　要：为了简化烟叶样品前处理步骤,采用正己烷直接萃取烟叶样品得到烟叶的正己烷萃取液,利用KOH水溶液对正己烷萃取液直接进行油水两相皂化,再采用高效液相色谱法测定皂化后萃取液中的茄尼醇浓度。结果表明：①萃取温度65℃,萃取时间24 h,KOH水溶液浓度0.01 mol/L,皂化时间0.5 h时,萃取效率较好,皂化转化率较高;②茄尼醇的质量浓度范围为2.125-34.000 mg/L时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99998;③平均回收率为97.6%,相对标准偏差（RSD）为2.7%（n=3）。表明该方法快速、简便、准确。In order to simplify the pretreatment of tobacco leaf samples, leaf sample was directly extracted by hexane and the obtained extract was saponified with potassium hydroxide, the solanesol concentration in saponified extract was determined by HPLC. The result showed that： 1） the efficiencies of extraction and saponification were better when the extraction lasted 24 hrs at 65 ℃ and the extract was saponified with 0.01 mol/L KOH for 0.5 hrs; 2） the peak area had good linear relationship with mass concentration in the concentration range of 2. 125 to 34. 000 mg/L （r = 0. 99998） ; 3） the average recovery was 97.6% and the relative standard deviation （RSD） was 2.7% （n =3）. The method was rapid, simple and accurate.

关 键 词：茄尼醇 高效液相色谱 烟叶 萃取 皂化 

分 类 号：TS411.1[农业科学—烟草工业]