薄荷总DNA导入烟草的变异后代研究  

Study on Hybrid Descendant of Tobacco Transformed by Mentha Total DNA Introduction

作　　者：李雪君[1] 段旺军[1] 李耀字[1] 马彩娟[3] 牛宝权[3] 崔红 

机构地区：[1]河南省农业科学院烟草研究中心,河南省许昌市461000 [2]国家烟草栽培生理生化研究基地,郑州市450002 [3]河南省烟草公司许昌分公司,河南省许昌市461000

出　　处：《烟草科技》2008年第3期53-55,62,共4页Tobacco Science & Technology

基　　金：国家烟草专卖局受助项目“花粉管通道法培育烟草新品系”(110200201002)

摘　　要：为了选育具有疗效的烟草新品种,采用花粉管通道法将薄荷的总DNA导入到香料烟栽培品种Basma中,其后代产生了广泛的变异。通过连续3年的筛选,从中选出性状较好的品系BD804。常规化学成分分析表明,后代与烟草差异不大;GC/MS检测表明,BD804中薄荷醇的含量远高于烟草。应用PCR技术对其进行了RAPD分析,结果表明78个引物在烟草、薄荷和BD804中共扩增出了668个条带,其中BD804中有14个为薄荷的特异性条带,后代和烟草的相似率为92.80%,与薄荷的相似率为5.84%,这说明薄荷DNA导入烟草引起了烟草基因组的变异。In order to breed new tobacco varieties with medicable function, the total DNA of medicinal plant mentha （Mentha haplocalyx Briq. ） was introduced into oriental tobacco cv. Basma via pollen-tubeway, the descendants variated comprehensively. A hybrid descendant assuming better traits, designated BD804, was obtained after screening for 3 consecutive years. Routine chemical component analysis indicated that there was no noticeable difference between BD804 and tobacco, and GC/MS test showed that the content of menthol in BD804 was far higher than that in tobacco. 78 primers were used to detect the genomes of tobacco, mentha and BD804 with RAPD. The results indicated that 668 bands were produced in tobacco, mentha and BD804, among them, 14 bands of BD804 were discovered only in mentha, and the similarity rates of BD804 with tobacco and mentha were 92.80% and 5.84% , respectively. It indicated that some DNA fragments of mentha were integrated into tobacco and tobacco genome variation resulted.

关 键 词：香料烟 薄荷 总DNA 变异后代 RAPD 

分 类 号：S572.035.3[农业科学—烟草工业]