抗血管内皮细胞抗体在结缔组织病相关肺动脉高压中的初步研究  

Prevalence of anti-endothelial antibody in patients with pulmonary arterial hypertension associated with Connective

作　　者：艾军[1] 李梦涛[1] 田庄[2] 方全[2] 郑文洁[1] 曾学军[1] 何建国[3] 曾小峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院风湿免疫科,100730 [2]中国医学科学院中国协和医科大学北京协和医院心内科,100730 [3]阜外心血管病医院肺血管病研究中心

出　　处：《中华风湿病学杂志》2008年第4期230-233,共4页Chinese Journal of Rheumatology

基　　金：国家科技支撑计划“十一五”重大项目（2006BA101A07）

摘　　要：目的研究抗血管内皮细胞抗体（AECA）在结缔组织病（CTD）相关肺动脉高压（PAH）患者血清中的检出情况，探讨AECA与CTD相关PAH临床特征的相关性。方法免疫印迹法检测39例CTD相关PAH患者、22例CTD不伴PAH患者及30名正常人血清中的AECA。结果CTD相关PAH患者血清AEcA检出率为82％，显著高于正常对照组（20％，P〈0.05）；与CTD组（73％）差异无统计学意义（P〉0．05）。CTD相关PAH患者血清AECA识别的主要抗原成分中相对分子质量22000的抗原仅出现在CTD相关PAH患者（15％）；抗相对分子质量75000抗原的AECA在CTD相关PAH患者中检出率为51％，显著高于CTD组（23％）及正常对照组。CTD相关PAH有雷诺现象患者中抗相对分子质量75000的抗原的AEcA检出率为63％，高于没有雷诺现象患者（40％），但差异无统计学意义（庠0．15）；抗核糖核蛋白（RNP）抗体阳性患者中抗相对分子质量75000抗原的AECA检出率为71％，显著高于抗RNP抗体阴性患者（28％，P〈0．01）。结论CTD患者血清的AECA有较高的检出率，抗相对分子质量22000和75000抗原的AECA可能在CTD相关PAH的发病中有一定作用。Objective To investigate the prevalence of anti-endothelial cell antibody （AECA） in the sera of patients with connective tissue diseases （CTD） complicated with pulmonary arterial hypertension （PAH） and to detect the specific antigens of AECA which may be related to clinical manifestations. Methods AECA was detected with Western blotting in 39 CTD patients with PAH, 22 CTD patients without PAH and 30 healthy donors. Results The prevalence of AECA was 82% in CTD patients with PAH, 73% in CTD patients without PAH and 20% in healthy donors. Anti-22 000 AECA was only detected in CTD patients with PAH （15%）. Anti-75 000 AECA was more frequently detected in CTD patients with PAH than in those without PAH （51% vs 23%, P〈0.05）. In CTD patients complicated with PAH, anti-75 000 AECA was more frequently detected in those with Raynaud＇ s phenomenon or with positive anti-RNP antibody. Conclusion AECA can be more frequently detected in CTD patients with or without PAH. Furthermore, anti-22 000 and anti-75 000 AECA may play a special role in CTD patients with PAH.

关 键 词：自身免疫病 高血压 肺性 抗内皮细胞抗体 印迹法 蛋白质 

分 类 号：R686[医药卫生—骨科学]