BMP-4基因／TGF—β1对兔火器性股骨缺损的治疗作用  

Gene therapy of firearm femur defect by using BMP-4/TGF-β1 in rabbits

作　　者：李兵仓[1] 张建军 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所,创伤、烧伤与复合伤国家重点实验室,重庆400042 [2]邯郸解放军第二八五医院药剂科,056001

出　　处：《中华创伤杂志》2008年第4期289-293,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（39970758）;全军“十五”指令性课题资助项目（01L060）

摘　　要：目的观察BMP-4基因联合TGF—β1对火器性股骨缺损的修复作用。方法用火器性钢珠造成兔股骨中段缺损，伤后2周于骨缺损上、下端和中部注射经体外鉴定的BMP-4基因和TGF—β1。用RT—PCR和Western blot检测BMP-4 mRNA与蛋自在体内的表达，用碱性磷酸酶（ALP）活性和钙含量检测局部组织的成骨能力，用病理学和X线检查成骨过程和质量。结果注射后BMP-4 mRNA和蛋白可在体内表达6周。实验组AIJP活性伤后8周时增加到（13．17±0．51）U／100ml，显著高于对照组的（8．77±0．44）U／100ml，提示实验组的成骨能力增强，钙含量检测与此相符。病理学和X线检查证明实验组成骨过程加快，成骨质量提高。结论BMP-4基因／TGF—β1对火器性骨缺损有明显的促修复作用。Objective To observe effects of BMP-4 gene combined with TGF-β1 in repairing rabbit femur defect resulted from firearm wound. Methods The femur defect was made with firearm steel ball. Plasmid encoded BMP-4 gene identified in vitro and TGF-β1 were injected into the tissue of upper, lower and central parts of the defects at the second week after wound. The mRNA and protein expressions of BMP-4 in vivo were detected by real time-polymerase chain reaction （RT-PCR） and Western blot. Activity of alkaline phosphatase （ALP） and calcium content were measured for evaluating osteogenetic ability. The process and quality of osteogenesis were determined by pathological and X-ray examinations. Results mRNA and protein of BMP-4 could continual/y express for six weeks in vivo after injection. Activity of ALP in the experimental group was increased to （13.17 ±0.51 ） U/100 ml at the 8th week, which was significantly higher than （ 8.77 ± 0.44） U/100 ml in the control group, indicating that osteogenetic ability was markedly enhanced, which accorded with determination of calcium content. Pathological observation and X-ray proved prominent improvement of osteogenesis, with a shorter time and better quality, in the experimental group. Conclusion BMP-4/TGF-β1 can promote repair of firearm femur defect.

关 键 词：骨形态发生蛋白-4 转化生长因子-Β1 创伤 撞击 骨缺损 

分 类 号：R683[医药卫生—骨科学]