应用人脐带血间充质干细胞修复小鼠皮肤缺损创面  

Repair of mouse skin wound by using mesenchymal stem cells isolated from human umbilical cord blood

作　　者：程文广[1] 黄正根[1] 贺伟峰[1] 袁顺宗[1] 陈希炜[1] 罗高兴[1] 吴军[1] 

机构地区：[1]第三军医大学附属西南医院烧伤研究所,重庆市疾病蛋白质组学重点实验室, 重庆400038

出　　处：《中华创伤杂志》2008年第4期298-301,共4页Chinese Journal of Trauma

基　　金：军队“十一五”国际合作项目（06H031）;军队“十一五”专题资助项目（06-Z-054）;重庆市自然科学基金资助项目（2005BB5044）

摘　　要：目的探讨人脐带血间充质干细胞（hUCBMSCs）在体分化为皮肤细胞、修复皮肤缺损创面的可能性。方法分离hUCBMSCs，体外培养、扩增并用BrdU标记后，种植于重症联合免疫缺陷（SCID）小鼠背部全层皮肤缺损创面（治疗组），并设同体PBS对照组。于术后7，14d观察并比较愈合率；于术后7，14，28d创面活检，行常规病理组织切片HE染色、免疫组织化学染色检测BrdU及人角蛋白19的表达。结果术后7，14d治疗组创面愈合率为（56．06±3．04）％和（94．75±1．89）％，对照组为（36．99±2．17）％和（84．63±2．28）％，治疗组高于对照组（P〈0．01）。HE染色显示治疗组活检组织表皮层明显增厚，细胞及表皮嵴数目显著增多；在再生表皮的毛囊内层以及表皮基底层、棘层可见BrdU阳性标记细胞连续分布，且有部分细胞表达人角蛋白19；并在治疗组活检组织中发现有皮肤附属器形成。结论hUCB MSCs可在体分化为皮肤细胞与组织，可应用于皮肤创面的修复。Objective To study the probability of applying mesenchymal stem cells isolated from human umbilical cord blood （hUCB MSCs） to repair mouse skin wound in vivo. Methods hUCB MSCs isolated from full term delivery human umbilical cord blood were cultured and amplified in vitro. hUCB MSCs at passage 9 were labeled with BrdU （5-bromodeoxy-uridine） and grafted on the full-thickness skin loss wound created on the back of the severe combined immunodeficiency （SCID） mouse （treatment group）, when a PBS control group was set. The wound healing rate was surveyed and compared at days 7 and 14 postoperatively. Meanwhile, the wound was biopsied at days 7, 14 and 28 after operation, and the expressions of BrdU antibody and K19 antibody were checked pathologically and immunohistochemically by HE staining, respectively. Results The wound in treatment group was healed more rapidly than that in control group （P 〈0.01 ）. The pathological check of the biopsy sample showed that the epidermis was thicker, with more epidermal ridges in the treatment group, compared with control group. It was found that some BrdU positive cells were distributed successively on the hair follicle, the stratum basal and the spinosum layers, a few of which even expressed K19. Conclusion hUCB MSCs can be differentiated into skin tissue and cells and is possible to repair skin wound.

关 键 词：脐带血  人 间充质干细胞 伤口愈合 细胞 表皮 重症联合免疫缺陷小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R641[医药卫生—基础医学]