大鼠皮肤和肌肉挫伤mRNA差异显示技术中总RNA的提取和检测  

Purification and checking of total RNA in rat skin and muscle contusion with differential display technology

作　　者：王小伟[1] 孙俊红[1] 杜秋香[1] 籍晓元[1] 王英元[1] 

机构地区：[1]山西医科大学法医学院,030001

出　　处：《山西医药杂志（上半月）》2008年第4期295-296,共2页Shanxi Medical Journal

基　　金：山西省留学回国人员基金资助项目(200528);山西省青年科技研究基金资助项目(2007021047)

摘　　要：目的研究大鼠皮肤和肌肉挫伤总RNA提取对差异显示技术的影响。方法使用Invitrogen TRIzol Reagent提取总RNA,由于皮肤与肌肉所含的蛋白比较多,所以改进了传统的提取步骤,可以减少杂质的残留。结果改进的Invitrogen TRIzol Reagent提取的RNA呈现28SrRNA,18S rRNA和5S rRNA3条较清晰的条带,其A260/A280值可达1.913,具有很高的纯度。结论大鼠皮肤和肌肉mRNA差异显示技术中,皮肤与肌肉总RNA的质量是十分关键的因素,通过对传统方法进行改进,建立起较完善的大鼠皮肤和肌肉mRNA差异显示技术体系,为开展损伤时间的分子鉴定与功能基因的分离、克隆奠定基础。Objective To investigate the effect of total RNA extraction from rat skin and muscle contusion on differential display technology. Methods Using Invitrogen TRIzol Reagent total RNA was extracted. The residual impurities can be reduced by improving the traditional extraction method in that the skin and muscle contain more protein. Results Improved Invitrogen TRIzol reagent RNA extraction showed more clear bands of 28S rRNA, 18S rRNA and 5S rRNA, and the A260/A280 value is up to 1. 913. Conclusion The quality of total RNA in rat skin and muscle is a crucial factor in differential display technology, by the improvement of traditional methods, a better differential display technology system is established for molecular identification and gene cloning of injury time.

关 键 词：大鼠 挫伤 差异显示 MRNA 

分 类 号：R346[医药卫生—基础医学]