在光动力光疗中类卟啉稀土配合物光敏作用原初反应的特征——产生自由基I型机制的ESR研究  

Characterization of the Initial Photosensitization of Porphyrin-like Rare-earth Metal Complex in Photodynamic Therapy——ESR Study of the Type I Mechanism of Free Radical Producation

作　　者：孙文芳[1] 董世明[1] 王夺元[1] 李美芬[2] 乐加昌[2] 万谦[2] 庞素珍[2] 

机构地区：[1]中国科学院感光化学研究所,北京100101 [2]中国科学院生物物理研究所,北京100101

出　　处：《化学学报》1997年第7期685-692,共8页Acta Chimica Sinica

基　　金：国家自然科学基金资助课题

摘　　要：本文用ESR方法研究了类卟啉稀土配合物[(CO_2H-APPC)Gd]Cl_2的光敏反应.用4-hydro-tetramethylpiperidine-N-oxide radical(4-hydro-TEMPO)作探针,通过对其消自旋的作用,证实[(CO_2H-APPC)Gd]Cl_2光敏反应中有阳离子自由基[(CO_2H-APPC)Gd]^(+)产生,加入还原剂可促使[(CO_2H-APPCGd]^+生成.经由5,5-Dimethyl-1-pyrroline N-oxide(DMPO)对超氧阴离子(O_2^-)和羟基自由基(·OH)的自旋捕捉及对该自旋加合物[DMPO-O_2^-]和[DMPO-OH]的ESR测定,证实有O_2^-和·OH产生,并用SOD清除O_2^-和甲酸钠清除·OH的实验,进一步证实O_2^-和·OH的产生.上述结果说明[(CO_2H-APPC)Gd]Cl_2光敏反应存在着产生[(CO_2H-APPC)Gd]^+和活性氧自由基的Ⅰ型机制.By means of ESR, we studied the photosensitization of porphyrin - like rareearth metal complex [ (CO2H - APPC)Gd]Cl2. By using 4 - oxo - TEMPO as a probe and through its spinelimination,the production of cation free radical [(CO2H-APPC)Gd]+in [(CO2H-APPC) -Gd]Cl2 photosensitization was established and moreover it might be promoted by using BNAH as areducing agent. Through trapping O2- and OH with DMPO and measuring the ESR spectra ofthe spin adducts of [DMPO - O2- ] and [DMPO - OH], the O2- and OH were found to be definitely generated. These results showed the presence of oxygen radicals in [(CO2H-APPC) -Gd]Cl2 photosensitization,and shed light on the type I mechanism.

关 键 词：类卟啉 稀土配合物 光敏反应 光疗药物 ESR 

分 类 号：R979.19[医药卫生—药品]