丁[鱼岁]遗传多样性的随机扩增多态性DNA分析  

Genetic Diversity Analysis of Tinca tinca by Random Amplified Polymorphic DNA Method

作　　者：杨太有[1] 陈宏喜[1] 关建义[1] 刘向奇[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2008年第2期111-113,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省动物学重点学科资助项目

摘　　要：利用RAPD分子标记技术对15个丁[鱼岁]养殖个体的遗传多样性进行分析.选用20个随机引物对其基因组DNA进行PCR扩增,有16个引物可以扩增出稳定且清晰的条带,其中S103、S104和S105为多态引物.16个引物共扩增出65个DNA位点,片段大小在200-3 000 bp之间,其中多态性位点6个,多态位点比例为9.23%.个体间遗传距离在0-0.067 4之间,平均遗传距离为0.028 6.结果显示,丁[鱼岁]养殖群体的遗传多样性水平较低.Randomly Amplified Polymorphic DNA （RAPD） is used to detect the genetic diversities of 15 cultured individuals of Tinca tinca. 16 RAPD primers are selected to amplify the genome DNA from 20 ones, three of which are polymorphic primers （S103, S104 and S105）. A total of 65 reproducible and stable bands, ranging from 200 to 3 000 bp,are amplified from 15 individuals. 6 of 65 loci detected are polymorphic, polymorphic loci is 9.23%. The genetic distance between individuals is from 0 to 0. 067 4, and the average genetic distance is 0. 028 6. In contrast to the genetic diversity in different species, the resuits show that the genetic diversity level of Tinca tinca is low.

关 键 词：丁[鱼岁] 遗传多样性 随机扩增多态性DNA 

分 类 号：Q341[生物学—遗传学]