刚果红褪色法测定血红蛋白  

Fading Spectrophotometic Method for the Determination of Hemoglobin with Congo Red

作　　者：王运生[1] 陈亚红[1] 田丰收[1] 周杰[1] 

机构地区：[1]周口师范学院化学系,河南周口466000

出　　处：《河南师范大学学报（自然科学版）》2008年第2期136-138,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省教育厅自然科学基金(2007150048)

摘　　要：在碱性介质中,H2O2可以氧化偶氮染料刚果红使之褪色,而过氧化物模拟酶——血红蛋白能够加速刚果红的褪色程度.据此建立了一种方便、快捷、灵敏的测定血红蛋白的催化动力学新方法.该方法测定的线性范围为5.0×10^-9-1.0×10-6mol·L^-1,检出限为1.5×10^-9mol·L^-1,表观摩尔吸光系数为1.8×10^5L·mol^-1·cm^-1.该方法简单、快速、灵敏,已成功的应用于尿液中血红蛋白含量的测定,结果满意.A simple spectrometric method has developed for the determination of hemoglobin based on it＇s catalytic activity on oxidation of Congo red in presence of hydrogen peroxide oxidation. The results indicate that hemoglobin concentration demonstrates good linear relationship in the range of 5.0 × 10^-9 - 1.0 × 10^-6 mol·L^-1 , and the apparent molar absorptivity ε490 is 1.8 × 10^5 L· mol^-1 · cm^-1 with a detection limit of 1.5× 10-9 mol· L^-1. This method has been applied to determine hemoglobin concentration in urine samples with satisfactory results.

关 键 词：血红蛋白 刚果红 过氧化氢 分光光度法 

分 类 号：O657.32[理学—分析化学]