大黄素洗脱可降解涂层支架的制作及其体内外实验研究  

Biodegradable Polymer Coated Emodin Eluting Stent in vitro and in vivo

作　　者：赵燕超[1] 龚飞荣[1] 葛均波[2] 程树军[1] 陈建定[1] 

机构地区：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237 [2]复旦大学附属中山医院上海市心血管病研究所,上海200032

出　　处：《华东理工大学学报（自然科学版）》2008年第2期242-246,共5页Journal of East China University of Science and Technology

基　　金：国家863项目(2002AA326110);上海市科委项目(044319213);上海市科委基金资助项目(05DJ14005)

摘　　要：以肝素化吗啉二酮苄酯与L-丙交酯的共聚物材料作为药物支架可降解涂层的药物载体材料,大黄素作为药物,在自制的计算机控制的喷涂设备上成功制备了大黄素药物洗脱支架(药物含量(150±5)μg)。并研究了大黄素支架在体外磷酸盐缓冲液(PBS,pH=7.4)中,(37±0.5)℃下的释放。最后在体内与裸支架和不含药的聚合物涂层支架进行了1个月和3个月的对照实验。研究发现,所制备的药物支架扩张后表面无撕裂、挂膜。此外,高含量比的药物支架(大黄素含量50%)中的药物10 d内的释放达到90%。3个月后体内涂层材料大部分发生降解,从血管切片和内膜厚度可以看出,大黄素对内膜增生有着明显的作用,可以抑制血管内的再狭窄。H eparinizing copolymer of L-lactide and （3S）-3-[ （Benzyl oxycarbonyl） methyl] morpholine- 2, 5-dione mixed with emodin as delivered drug was applied to the bare metal stents with self-made spry instrument to prepare the emodin eluting stents （Emodin （150 ± 5）μg）. Emodin released from the stent was investigated in PBS at （37±0.5）℃. The in vivo experiments were proceeded from 1 to 3 months with polymer coating stent and bare metal stent as the control groups. SEM images showed that the surfaces of expanded emodin eluting stents were crackless. In addition, the in vitro release investigation indicated 90% of emodin had been released within 10 days in terms of drug-eluting stents with high emodin content coating （Emodin 50%）. According to the in vivo experiments in pigs, the coating degraded almost entirely in 3 months. The results of section pictures and neointimal thickness show emodin could suppress the neointimal hyperplasia and the restenosis effectively.

关 键 词：肝素化共聚物 大黄素洗脱支架 再狭窄 体内实验 可降解涂层 

分 类 号：TQ32[化学工程—合成树脂塑料工业] R318[医药卫生—生物医学工程]