大豆疫霉菌、水杨酸和创伤诱导的拟南芥蛋白质谱的比较研究  

Comparative Proteomics of Arabidopsis Induced by Salicylic Acid, Wound and Phytophthora sojae

作　　者：孙果忠[1] 朱振东[1] 武小菲[1] 崔友林[1] 王晓鸣[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《中国农业科学》2008年第4期1030-1039,共10页Scientia Agricultura Sinica

基　　金：国家重点基础研究发展计划"973"项目(2002CB111406-6)

摘　　要：【目的】系统获得抗性(system acquired resistance,SAR)是植物抵抗病原菌侵染的一种复杂机制,本研究旨在鉴定大豆疫霉菌(Phytophthora sojae)诱导的拟南芥系统获得抗性相关的蛋白质。【方法】利用双向电泳分离创伤、水杨酸(Salicylic acid,SA)和大豆疫霉菌诱导的拟南芥叶片的总蛋白,并对28个差异表达的蛋白点进行了MALDI-TOF质谱鉴定。【结果】这28个蛋白点的表达模式可分为3种类型:13个点的表达随着伤口、SA和P.sojae的诱导而上调;11个点的表达随着伤口、SA和P.sojae的诱导而下调;4个点在伤口和P.sojae处理中的表达行为与SA处理相反。这些蛋白质涉及了物质代谢酶、调节因子或分子伴侣、氧化还原酶、防御相关蛋白、蛋白激酶、叶绿体蛋白和功能未知蛋白等复杂的生物学功能。【结论】创伤、SA和P.sojae诱导的拟南芥系统获得抗性涉及的抗性组分有很大的重叠,在信号传导网络上也存在一定的交叉。蛋白质组分析为研究复杂的植物抗性机制提供了可行的技术手段。[Objective ] System acquired resistance （SAR） is a complex disease resistance mechanism in plants. The study was aimed at elucidating the protein profiles of the SAR of Arabidopsis induced by Phytophthora sojae. [Method] Total proteins prepared from Arabidopsis leaves induced by wound, salicylic acid （SA） and P. sojae were isolated by 2D PAGE. A total of 28 spots differentially expressed between the CK and treatment were identified by MALDI-TOF mass spectrometric analysis. [Result] Among them, 13 spots were up regulated and 11 spots were down regulated in the treated leaves; interestingly, the expression patterns of 4 spots in leaves induced by SA were opposite to that of wound and P. sojae. Functions of these proteins could be classified as metabolic enzymes, chaperone, oxidoreductase, defense-related proteins, protein kinase, chloroplast proteins and unknown function proteins. [Conclusion] Studies of expression pattems of these proteins have provided new information on the overlap of defense element and cross-communicating signal transduction pathways in SAR between wounding, SA and P. sojae attack. Proteome analysis is a useful approach to study the complex resistance mechanism in plants.

关 键 词：拟南芥 大豆疫霉菌 水杨酸 系统获得抗性 蛋白质组学 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S511.032[农业科学—植物保护]