小菜蛾nAChR靶标敏感性与沙蚕毒素类药物的抗性关系  

Target Sensibility of Nerve Acetylcholine Acceptor and Nereistoxin Insecticides Resistance in Diamondback Moth, Plutella xylostella (L.)

作　　者：程罗根[1] 于光[2] 李忠英[3] 

机构地区：[1]南京师范大学生命科学学院生物多样性与生物技术江苏省重点实验室,南京210097 [2]南京中医药大学基础医学院,南京210046 [3]贵州省农业科学院植保所,贵阳550006

出　　处：《中国农业科学》2008年第4期1048-1052,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30160050)

摘　　要：【目的】研究抗杀虫双、杀螟丹和溴氰菊酯品系的神经乙酰胆碱受体及与它们对沙蚕毒素类药物的抗性间的关系。【方法】测定不同品系小菜蛾的神经乙酰胆碱受体与125I标记的α-银环蛇毒素的结合率。【结果】抗杀虫双、杀螟丹品系小菜蛾的神经乙酰胆碱受体对沙蚕毒素药物有显著的靶标不敏感性,它们与配体的结合率大约分别是敏感品系的66%和60%,在溴氰菊酯抗性品系中没有明显变化。【结论】神经乙酰胆碱受体对杀虫剂敏感性下降可能是小菜蛾对沙蚕毒素药物产生抗性的一种作用机理。[Objective] The relations between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella （L.）, were analysed. [ Method ] The combinative rate measurement of （3-[125I] iodotyrosyl） α-bungarotoxin was applied in the analysis. [Result] In the dimehypo resistant strain and the cartap resistant strain, the nerve acetylcholine receptor shown remarkable insensitivity to dimehypo and cartap, their binding rate to ligand was about 66% and 60% of the susceptible strain, respectively. [Conclusion] The sensitivity to deltamethrin in the deltamethrin resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.

关 键 词：小菜蛾 杀虫双 杀螟丹 神经乙酰胆碱受体 抗药性 

分 类 号：S482.3[农业科学—农药学]