猪带绦虫新基因SLC10的研究  

Research of SLC10 –a New Gene from Taenia solium

作　　者：王颖[1,2] 骆学农[1] 郑亚东[1] 丁军涛[1] 侯俊琳[1] 张少华[1] 景志忠[1] 才学鹏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,兰州730046 [2]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《中国农业科学》2008年第4期1213-1218,共6页Scientia Agricultura Sinica

基　　金：国家重大基础研究发展规划“973”项目(G1999011906)

摘　　要：【目的】克隆和表达猪囊尾蚴SLC10基因,明确其组织分布,为寻找猪囊虫病的新型疫苗或诊断候选基因奠定基础。【方法】以绦虫保守的反式剪接引导序列为基础,通过RT-PCR从猪囊尾蚴中克隆出来的一个未知基因,将其在大肠杆菌中表达,用亲和层析纯化的重组蛋白作为包被抗原进行ELISA检测和免疫组织化学试验。【结果】将新基因命名为SLC10,其ORF为507bp,编码18.2kD的蛋白,基因组全长1107bp,由两个外显子和一个内含子组成。ELISA结果显示70份囊尾蚴阴性血清和75份囊尾蚴阳性血清都不与重组蛋白发生反应,免疫组织化学实验表明SLC10天然蛋白主要分布在除猪囊尾蚴头节以外的囊壁内部。【结论】SLC10蛋白为非分泌型结构蛋白,与诱导宿主产生抵抗囊尾蚴感染的体液免疫应答无关。[ Objective ] Cloning, expression and immunohistochemistry of SLCIO gene in Taenia solium are not only helpful to us to understand the biological characteres of Taenia solium but also helpful to us to find the vaccine or diagnostic candidates. [Method] The unknown gene was cloned by spliced leader-based PCR from Taenia solium. The recombinant SLC10 protein was expressed in E. coli in a fusion pattern and purified by affinity chromatography. Using purified recombinant SLC 10 protein, ELISA and immunohistochemistry tests were made. [ Result ] The new gene was named SLCIO. The ORF of SLC 10 was found to be 507 bp, encoding an 18.2 kD protein. The genomic DNA of SLCIO was found to be 1 107 bp consisted of two exons and one intron. ELISA results showed that all detected sera, including 70 normal and 75 cysticercosis sera, did not react with purified recombinant SLC10 protein. Immunohistochemistry showed that the native SLC 10 protein distributed mainly in inner cyst walls but not in the scolex in C cellulosae. [ Conclusion ] Together with predicted results, it is suggested that the SLC 10 protein is a non-secretory structural protein, not involved in induction of the host＇s immune reactions against infection at least at the larval stage.

关 键 词：猪带绦虫 剪切引导序列 SLC10 

分 类 号：S852.7[农业科学—基础兽医学]