印楝素不同处理方法对烟粉虱的室内毒力测定  

Toxicity of Foliar or Systemically Applied Azadirachtin to Bemisia tabaci Gennadius (Homoptera, Aleyrodidae)

作　　者：文吉辉[1,2] 侯茂林[1] 卢伟[1,2] 黎家文[3] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室 [2]湖南农业大学生物安全科技学院,长沙410128 [3]湖南农业大学生物安全科技学院

出　　处：《中国农业科学》2008年第4期1242-1247,共6页Scientia Agricultura Sinica

基　　金：中央科研院所社会公益研究专项项目(2004DIB4J156)

摘　　要：【目的】明确印楝素及其不同施药方式对烟粉虱各虫态的致死作用。【方法】采用黄瓜叶片浸液法和叶柄内吸法测定了0.3%印楝素乳油对烟粉虱成虫、1日龄卵、5日龄卵和1～4龄若虫的室内毒力。【结果】在所测试的浓度范围(0.3125～10.0mg·L-1)内,两种处理方式下烟粉虱各虫态的校正死亡率均随印楝素浓度的增大而顺次显著增大,印楝素叶片浸液处理的校正死亡率高于叶柄内吸处理。印楝素叶片浸液处理对烟粉虱成虫、1日龄卵、5日龄卵、1龄若虫、2龄若虫、3龄若虫、4龄若虫的致死中浓度LC50值分别为3.0246、2.2019、5.7109、0.5909、0.7465、1.6948和2.6603mg·L-1,而叶柄内吸处理的LC50值分别为6.016、4.0321、6.0537、0.8614、0.9941、2.2419和4.4533mg·L-1。【结论】印楝素叶片浸液处理对烟粉虱各龄若虫的致死作用均高于叶柄内吸处理,烟粉虱低龄若虫期是采用印楝素防治的最佳时期。印楝素乳油可望成为防治烟粉虱的重要药剂之一。[Objective ] The objective of this experiment is to test mortality and LC50 of 0,3% azadirachtin （AZ） EC against sweetpotato whitefly, Bernisia tabaci. [ Method] Both cucumber leaf disc dipping method and petiole wrapping method were used, [Result] Mortalities of whiteflies treated by AZ disc dipping method were higher than that treated by petiole wrapping method at the tested AZ concentrations between 0.3125 mg.L^-1 and 10.0 mg·L^-1, and the mortalities of whiteflies in both the two bioassay methods were significantly dosage-dependent, The medium lethal concentration （LC50） of AZ by disc dipping method to adults, 1-d and 5-d old eggs and, 1st, 2nd, 3rd and 4th instar nymphs were 3.0246, 2.2019, 5.7109, 0.5909, 0.7465, 1.6948 and 2.6603 mg.L1, respectively; and the LCso of AZ treated by petiole wrapping method were 6.016, 4,0321, 6,0537, 0.8614, 0,9941, 2.2419 and 4,4533 mg·L^-1, respectively. [ Conclusion ] Therefore, lethal effect of dipping method is higher than wrapping application; young nymph is the best stage for whitefly control by AZ. AZ may be one of the most significant botanical chemical in the control of sweetpotato whitefly.

关 键 词：印楝素 烟粉虱 浸液生测法 内吸生测法 室内毒力 

分 类 号：S482.3[农业科学—农药学]