通心络治疗缺血再灌注损伤机制的脑片研究  

Effect and mechanism of Tongxinluo on ischemia/reperfusion injury through brain slices

作　　者：蒋海山[1] 李求实[1] 陆兵勋[1] 王立新[1] 

机构地区：[1]南方医科大学南方医院神经内科,广州510515

出　　处：《广东医学》2008年第4期536-538,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30400612)

摘　　要：目的通过器官型脑片研究通心络含药血清对缺血再灌注损伤的治疗作用及机制。方法制备生后3d SD的全脑器官型脑片,随机分为缺血再灌注脑片模型组和正常对照组,各组根据干预方式的不同再分为大剂量含药血清组、小剂量含药血清组、对照血清组、空白组,于干预后不同时间通过倒置显微镜及免疫荧光染色观察通心络对脑片上神经干细胞增殖、迁移、分化的影响。结果经含药血清干预后室下区、海马齿状回单位区域新生细胞(nestin+)数量增多,正常对照组新生细胞密度高于缺血再灌注脑片模型组(P<0.05);两组1,3,7d时新生细胞数量均按照大剂量含药血清组、小剂量含药血清组、对照血清组、空白组顺序递减(P<0.05);因新生细胞向外迁移,随着培养时间延长上述区域新生细胞密度逐渐减少;1d时各组均有大量nestin+新生细胞,7d时只有大、小剂量含药血清组有少量nestin+新生细胞。结论通心络可能通过促进神经干细胞的增殖迁移和分化来治疗缺血再灌注损伤,其效应随剂量增加而增强。Objective To explore the study and mechanism of Tongxinluo on ischemia/reperfusion injury through organotypic brain slices. Methods Brain slices （300μm） from postnatal 3^rd day SD rats were prepared and randomly divided into 2 groups, ischemia/reperfusion group and normal group. After ischemia/reperfusion injury, they were divided into 4 groups （high - dose Tongxinluo group, low - dose Tongxinluo group, control group and normal saline group）. Slices were observed under invert microscope or detected by immunofluorescence staining. Results Neonatal cells were labeled with nestin in the subventrical zone and hippocampal dentate gyrus when brain slices were treated with different dosage of Tongx- inluo, while ischemia/reperfusion group had less cells （P 〈 0.05 ）. After cultured for 1 day, 3 days and 7 days, the number of neonatal cells decreased in the order of high - dose Tongxinluo group, low - dose Tongxinluo group, control group and normal saline group （P 〈0. 05）. The density of neonatal cells in the subventrical zone and hippocampal dentate gyrus decreased due to cell migration with time. Each group had many neonatal cells labeled with nestin at 1st day, but only two Tongxinluo groups had some neonatal cells labeled with nestin at 7th day. Conclusion Tongxinluo can be used to treat is-chemia/reperfusion injury by inducing the proliferation, migration and differentiation of neural stem cells in a close - de- pendent wanner.

关 键 词：器官型培养 脑片 通心络 神经干细胞 细胞分化 

分 类 号：R657.3[医药卫生—外科学] R285.5[医药卫生—临床医学]