叶酸对胎鼠大脑神经干细胞增殖蛋白表达影响  

Effects of folate on proliferation of neural stem cells of fetal rat

作　　者：王广雷[1] 黄国伟[1] 张绪梅[1] 丛革新[1] 姬长珍[1] 田志红[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070

出　　处：《中国公共卫生》2008年第4期462-464,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金项目(30571563)

摘　　要：目的研究叶酸对胎鼠大噙神经干细胞（NSCs）增殖的影响及其作用机制。方法采用孕14～16dSD胎鼠，进行NSCs原代培养。将NSCs分为4组：对照组、叶酸缺乏组、叶酸低剂量组、叶酸高剂量组。收集培养6d的细胞，采用5-溴脱氧尿苷（BrdU）掺入法和免疫组化双标记检测NSCs的增殖情况，采用蛋白印迹法（Westernblot）检测胎鼠增殖期NSCs活化的细咆外信号调节激酶1／2（pERKl／2）的表达含量。结果叶酸低剂量组、高剂量组BrdU＋巢蛋白（Nestin）阳性细胞占总细胞（70．5±2．7），（78．8±6．9）％与对照组（64．2±8．9）％比较，差异均有统计学意义，且高剂量组明显高于低剂量组（P〈0．05）；叶酸低剂量组、高剂量组pERKl蛋白的表达（0．407±0．018），（0．443±0．019）与对照组（0．388±0．011）比较，差异均有统计学意义（P〈0．05）。叶酸低剂量组、高剂量组pERK2蛋白的表达（0．547±0．044），（0．563±0．041）与对照组（0．507±0．018）比较，差异均有统计学意义（P〈0．05）。结论叶酸能够促进胎鼠NSCs的增值。其作用机制可能是通过丝裂原活化蛋白激酶信号通路调节NsCs的增殖。Objective To study the effect of folate on neural stem cells（NSCs） proliferation via MARK signal pathway in fetal rats in vitro. Methods NSCs of fetal Sprague - Dawley rat from E14d to E16d were cultured, divided into four groups which were control group, folate - deficiency group, folate - low group and folate - high group. NSCs were collected af- ter being cultrured 6 d. The proliferation of NSCs was detected by BrdU in corporating method and immunohistochemistry method. The level of pERK1/2 expression were detected by western blot method. Results The percentage of Nestin and BrdU positive cells in total cells（x ± s ） in folate - low group（70.5 ± 2.7） and folate - high group （78.8 ± 6.9） was significantly higher than that in control group [ （64.2 ± 8.9）, P 〈 0. 053 . The levels of pERK1 （x ± s ） in folatelow group（0. 407 ± 0.018） and folate- high group （0.443 ± 0.019） were higher than those in control group（（0.388 ± 0.011）, P〈0.05）, and the levels of pERK2 in folate- low group （0. 547 ± 0. 044） and folate - high group （0. 563 ± 0. 041 ） were higher than those in control group （（ 0. 507 ± 0. 018 ）, P 〈 0.05 ）. Conclusion Folate could promote proliferation of fetal NSCs via MAPK signal pathway.

关 键 词：叶酸 神经干细胞 增殖 丝裂原活化蛋白激酶(MAPK)信号通路 

分 类 号：R532.1[医药卫生—内科学]