体外共培养双份脐血CD34^+细胞对各自归巢相关分子表达的影响  

Influence of Co-culture Ex Vivo of CD34^+ Cells from Different Two Units of Cord Blood on their Homing-related Adherent Molecules Expression

作　　者：姚雯[1] 汪健[1] 孙自敏[1] 刘会兰[1] 耿良权[1] 王兴兵[1] 

机构地区：[1]安徽医科大学附属省立医院血液科,安徽合肥230001

出　　处：《中国实验血液学杂志》2008年第2期368-372,共5页Journal of Experimental Hematology

基　　金：安徽省2006年度科技攻关项目;编号06013128B

摘　　要：本研究探讨脐血CD34+细胞体外混合培养对彼此归巢相关分子表达的影响。从正常人骨髓中分离扩增间充质干细胞,富集传代,加速器照射(12Gy)后作为滋养层细胞,将免疫磁珠分选纯化的两份脐血CD34+细胞混合接种到其表面(其中1份CD34+细胞用CFSE标记),观察各自归巢相关分子(黏附分子)表达的变化。结果表明,脐血CD34+细胞分选富集的纯度为(98.25±0.93)%,实验组在共培养6天后,两份脐血CD34+细胞的比例分别降为(60.4±6.32)%和(60.2±5.12)%,但与对照组比较无统计学差异;实验组各份CD34+细胞的CD44,CD62L,CD184以及CD26的阳性率较培养前均无显著变化。而CD162表达显著下降。CD54在培养3天后有所上升,但培养6天后下降至培养前水平,与对照组比较无统计学差异。结论:将两份脐血的CD34+细胞体外混合培养对彼此归巢相关分子并无明显影响。The study was aimed to explore the influence of co-culture ex vivo of CD34^＋ cells from two units of cord blood （ CB ） on the homing-related adherent molecule expression of each other. Mesenchymal stem cells （MSCs） were obtained from human bone marrow. Two units of CB CD34^＋ cells were co-cultured on 12 Gy γ-ray irradiated MSC layer. Their adherent molecule expressions were assessed by flow cytometry. The results showed that the purity of the isolated CD34^＋ cells was （98.25 ± 0.93 ） %. After co-culture on MSC layer for 6 days, the proportion of CD34^＋ cells of each unit was dropped to （60.4 ± 6.32 ） % and （60.2 ± 5.12） % respectively, but there was no significant difference from the control groups. The expressions of CD44, CD62L, CD184 and CD26 on CD34^＋ cells of each unit remained unaffected. The expression of CD162 was downregulated and CD54 was first increased but then dropped to the level before co-culture. But there was no significant difference between the experimental and control groups. In conclusion, co-culture of CD34^＋ cells from two units of CB may have no effects on the adherent molecule expressions of each other.

关 键 词：脐血 CD34^+细胞 体外共培养 归巢 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R331.1[医药卫生—基础医学]