肝细胞生长因子基因C57488A多态性与原发性高血压的关系  

Hepatocyte growth factor gene C57488A polymorphism and essential hypertension

作　　者：孟影[1] 王邦宁[1] 

机构地区：[1]安徽医科大学第一附属医院心内科1病区,安徽省合肥市230022

出　　处：《中国组织工程研究与临床康复》2008年第11期2043-2046,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：安徽省科技攻关计划项目(06013129B)~~

摘　　要：目的:血管内皮细胞功能不全在高血压患者中普遍存在,肝细胞生长因子在内皮细胞功能不全中起重要作用,与高血压的流行、严重程度、靶器官受损等有关。探讨肝细胞生长因子基因C57488A的多态性在原发性高血压发病机制中的作用。方法:①试验对象:选择2006-06/12在本院保健中心体检或本科室住院的原发性高血压患者共107例作为原发性高血压组,平均年龄(61±9)岁,其中有高血压家族史者共47例;所有对象均为合肥地区居住且无血缘关系的汉族人,患者对试验知情同意,试验经医院医学伦理委员会批准。病例收集按照中华医学会制订的高血压指南标准,收缩压≥140mmHg,或舒张压≥90mmHg(1mmHg=0.133kPa),或明确诊断为高血压而接受药物治疗达1年以上者。1个月内仅服用降压药。同时选择未曾接受高血压药物治疗的健康人共110名作为正常对照组,平均年龄(60±8)岁;纳入标准:收缩压<140mmHg,舒张压<90mmHg,并排除高血压家族史。所有受试对象均详细调查家族史,排除继发性高血压、糖尿病、冠心病、甲状腺疾病、严重的肝肾疾病及心肺功能不全等。②试验方法及评估:所有受试对象均测定血压、身高、体质量,计算出体质量指数,常规生化测定总胆固醇、三酰甘油、极低密度脂蛋白、空腹血糖等。采用单一等位基因特异性引物PCR技术测定两组肝细胞生长因子的基因型及肝细胞生长因子C57488A基因多态性分布、各基因型和等位基因频率分布。结果:纳入原发性高血压患者107例和健康人110名,均进入结果分析。①原发性高血压组和正常对照组的年龄、性别、体质量指数、三酰甘油、总胆固醇、极低密度脂蛋白和空腹血糖差异均无显著性(P>0.05)。②所有受试对象基因多态性分布符合Hardy-Weinberg平衡,具有群体代表性。原发性高血压组中有家族史者(47例)和无家族史者(60例)的CC、CA�AIM： The impaired function of vascular endothelial cells occurs popularly in hypertensive patients. Hepatocyte growth factor （HGF） plays a crucial role on impaired function, and is related to prevalence, severity and target organ damages of hypertension. This study explored the relationship between HGF gene C57488A polymorphism and essential hypertension （EH）. METHODS： OBetween June and December in 2006, totally 107 EH patients with an average age of （61 ±9） years, in them 47 cases with family history of hypertension, were recruited in this study. Informed consents were obtained from the patients who were all the Han residents of Hefei area, and the experiment was approved by the hospital ethical committee. The hypertension criteria formulated by Chinese Medical Association included the systolic pressure ≥140 mm Hg or diastolic pressure ≥ 90 mm Hg （1 mm Hg=0.133 kPa）, or having received drug therapy for hypertension over one year. The patients were administrated with hypotensive drugs with one month. Meanwhile 110 normotensives without family history of hypertension and drug therapy were served as controls, with an average age of （60±8） years. Inclusion crteria： systolic pressure 〈 140 mm Hg or diastolic pressure 〈 90 mm Hg （1 mm Hg=0.133 kPa）. All the subjects were surveyed for family history, excluding secondary hypertension, diabetes, coronary heart disease, thyroid disease, severe liver and kidney disease, cardiorespiratory function insufficiency and so on.②Detection parameters included blood pressure, height, weight, body mass index, and total cholesterol, triacylglycerol, very low density lipoprotein, fasting blood glucose were detected biochemically. Single allele-special primer polymerase chain reaction technique was used to examine the polymorphism of HGF genetypes and distribution of allele frequency in two groups. RESULTS： Both 107 EH patients and 110 healthy controls were involved in the result analysis.①There were no significant differences in age, se

关 键 词：肝细胞生长因子 原发性高血压 基因多态性 

分 类 号：R392.114[医药卫生—免疫学]