云南大理白族人群乙型肝炎病毒基因型和亚型的分布  

Distribution of hepatitis B virus genotypes and subgenotypes among Bai nationality in Dali,Yunnan Province

作　　者：李威[1] 张烜榕[1] 申元英[1] 任来峰[2] 沈茹[1] 李强[1] 赵海平[1] 

机构地区：[1]大理学院微生物学与免疫学教研室,云南大理671000 [2]山西医科大学汾阳学院微生物学与免疫学教研室,山西汾阳032200

出　　处：《中国病原生物学杂志》2008年第3期166-169,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金资助项目(No30560136);云南省中青年学术技术带头人后备人才培养基金项目(No2006py01-57)

摘　　要：目的调查云南大理地区白族人群乙型肝炎病毒(hepatitis Bvirus,HBV)基因型和亚型分布情况。方法在大理地区选择100例白族慢性HBV感染者,采用基因型特异性引物分型法和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定HBV感染者血清HBV基因型和亚型,并用ELISA法测定HBV血清标志物。结果100例HBV感染者中,B型41例(41%),全部为Ba亚型,未发现Bj亚型;C型25例(25%),其中Cs亚型21例(84%),Ce亚型3例(12%),未分型1例(4%);B+C混合型34例(34%)。B型感染者与C型感染者比较血清HBeAg阳性率差异有统计学意义(P=0.024)。结论云南大理白族人群HBV感染的基因型主要为B型、B+C混合型和C型,以Ba和Cs亚型为主要流行株,B+C混合型感染占很大比例。Objective To investigate the distribution of hepatitis B virus （HBV） genotypes and subgenotypes among the population of Bai nationality in Dali, Yunnan Province. Methods 100 patients with chronic HBV-infection were enrolled from Bai nationality in this study. HBV genotypes and subgenotypes were determined by genotype-specific primers and PCR-RLFP, respectively. The HBV markers, including HBsAg, HBsAb, HBeAg, HBeAb and HBcAb, were tested with ELISA. Results Among 100 patients with chronic HBV infection, the proportions of genotype B, C and B＋C were 41%, 25% and 34% respectively. All genotype B strains belonged to subgenotype Ba and subgenotype Bj was not found. In genotype C, 21（84%） weresubgenotypeCs, 3（12%） weresubgenotypeCe, and the remainingl（4%） was not indentified by PCR-RLFP. There were statistically significant differences in the distribution of HBeAg positive rates between patients with genotype B and those with genotype C （P=0. 024）. Conclusion Genotype B, mixed genotype （B ＋C） and genotype C existed among Bai nationality with chronic HBV-infection in Dali and mixed genotype （B＋C） accounted for a high proportion. Subgenotype Ba and Cs were predominant strains in this district.

关 键 词：肝炎病毒 乙型 基因型 亚型 聚合酶链反应 限制性片段长度多态性 

分 类 号：R373.21[医药卫生—病原生物学]