外搽非光敏中药组方对大鼠表皮c-KIT和PAR-2蛋白表达的促进作用  

Effects of Extracts from Non-photosensitive Traditional Chinese Medicinal Herbs on the Expression of c-KIT and PAR-2 protein in Rat Epidermis

作　　者：阮高波[1] 李永伟[1] 许爱娥[1] 

机构地区：[1]杭州市第三人民医院皮肤科,浙江杭州310009

出　　处：《中华中医药学刊》2008年第4期806-808,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：杭州市科技局资助项目(2001123833)

摘　　要：目的:探讨非光敏中药组方中药2号临床治疗白癜风有效的可能机制。方法:中药2号乙醇提取液外搽大鼠背部皮肤,并以75%乙醇外搽作为阴性对照,4周后取组织活检。采用免疫组化检测大鼠表皮中S100、干细胞因子受体(c-K IT)和蛋白酶激活受体-2(PAR-2)表达量的变化。结果:与75%乙醇外搽相比,中药2号外搽后S100阳性细胞数无显著性差异(P>0.05);c-K IT和PAR-2阳性细胞数均有增加,差异有显著性(P<0.05)。讨论:中药2号外搽治疗白癜风有效的机理可能与促进细胞移行、黑素转运有关;而与黑素细胞增殖无关。Objective ：To explore the possible mechanism of extracts of non - photosensitive traditional Chinese Medicinal Herbs （ No. 2 Traditional Chinese Medicinal Herbs, No. 2 TCMHs ） in the treatmentof vitiligo. Methods ： No. 2TCMHs were extracted by alcohol, then the rat skin was treated by the ethanol extracts twice a day, and ethanol was used as negative control. Four weeks later,the skin specimens were obtained. Immunohistochemistry method was used to detect the S100, Kit - tyrosine kinase receptor（ c - KIT） and proteinase - activated receptor - 2 （ PAR - 2 ） protein expression in these specimens. Results ：Compared with the ethanol group, there was a significant increase in the positive rates of c - KIT and PAR - 2 expression in No. 2TCMHs treated group （ P 〈 0. 05 ）, while there was no significant increase of the S100 positive cells （ P 〉 0.05 ）. Conclusion ： Our results suggest that the efficacy of No. 2TCMHs in the treatment of viti- ligo may be related to its ability to enhance cell migration and melanin transfer,and not related to the increasing of mela- nocytes.

关 键 词：中草药 非光敏 c—KIT PAR-2 大鼠 白癜风 

分 类 号：R285.5[医药卫生—中药学]