大孔吸附树脂分离纯化荷叶中的荷叶碱  

Separating and Purifying the Nuciferine of Lotus Leaf by Macroporous Adsorptive Resins

作　　者：封聚强[1] 时书杰[1] 赵骏[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《中华中医药学刊》2008年第4期877-879,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：优化大孔吸附树脂分离纯化荷叶中荷叶碱的洗脱条件。方法：用D101大孔吸附树脂进行分离纯化，20％～70％的乙醇梯度洗脱，并采用HPLC对其中的荷叶碱进行含量测定。结果：采用50％～70％的醇梯度洗脱后荷叶碱的总收率达1．3％。结论：此方法操作简单，重复性好，用50％～70％的乙醇进行梯度洗脱，能有效的提纯、富集荷叶中的荷叶碱。Objective ：To optimizing the elution requirement of separating and purifying the nuciferine of lotus leaf by Macroporous adsorptive resins. Methods ： D101Macroporous adsorptive resins was adopted to separate and purify, gradient elution with 20%-70% alcohol,and HPLC was adopted to undertake assaying of the nuciferine. Results .Total yield of the nuciferine by adopting the gradient elution with 50%-70% alcohol arrives 1.3%. Conclusion ：This method is simple to operate and has good reproducibility. It can purify and enrich the nuciferine of lotus leaf effectively by the gradient elution with 50%-70% alcohol.

关 键 词：荷叶碱 D101大孔吸附树脂 梯度洗脱 含量测定 

分 类 号：R284.2[医药卫生—中药学]