烹调油烟颗粒物对HELF细胞的氧化应激效应研究  

Oxidative Stress on Human Embryonic Diploid Lung Fibroblasts Induced by Cooking Oil Fume Particulate

作　　者：于仲波[1] 陈琼姜[1] 谭玉凤[1] 金锋[1] 柴剑荣[1] 陶核[1] 金勇[1] 吴南翔[1] 

机构地区：[1]浙江省医学科学院卫生学研究所环境医学研究室,杭州310013

出　　处：《癌变．畸变．突变》2008年第2期127-130,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：浙江省医药卫生重点科技项目(2002ZD002)

摘　　要：背景与目的:探讨烹调油烟颗粒物(cooking oil fume particulate,COFP)对人胚肺二倍体成纤维细胞(human embryonic diploid lung fibroblasts,HELF)的氧化应激效应。材料与方法:用玻璃纤维滤膜采集烹调油烟颗粒物,通过MTT实验确定COFP暴露对HELF细胞的IC50。将HELF细胞暴露于20、4、0.8μg/ml的COFP,分别于12、24、48h后进行活性氧(reactive oxygen species,ROS)分析、丙二醛(malondialdehyde,MDA)检测和彗星试验(cometassay)。结果:COFP作用12、24、48h后HELF细胞的IC50分别是101.7、82.7、85.1μg/ml,细胞质内ROS平均荧光强度随COFP剂量增加而增高,在COFP0.8、4μg/ml暴露24、48h后线粒体内的ROS较阴性对照组升高,其差异具有统计学意义(P<0.05)。实验组MDA水平与阴性对照组相比差异无统计学意义(P>0.05)。实验组DNA断裂水平与阴性对照组相比,差异有统计学意义(P<0.05)。各实验组引起的细胞ROS、MDA升高和DNA断裂在不同暴露时间之间差别均无统计学意义(P>0.05)。结论:在实验剂量水平和暴露时间内,COFP暴露可引起HELF细胞胞质和线粒体内ROS升高、DNA断裂,但并不能引起脂质过氧化损伤。BACKGROUND AND AIM： Oxidative stress effect induced by cooking oil fume particulate （COFP） on human embryonic diploid lung fibroblasts （HELF）was investigated. MATERIALS AND METHODS： Glass fibre filter film was used to collect COFP. IC50 was determined by MTr assay. HELF cells were exposed to COFP at the doses 20, 4, 0.8 μg/ml for 12, 24 and 48 hours. Then ROS analysis, MDA test and comet assay were performed, RESULTS： The IC50 for 12, 24, 48 hours was 101.7 μg/ml, 82.7 μg/ml and 85.1 μg/ml, respectively. The mean cytoplasmic fluorescence intensity increased as the COFP dose increased. Significant difference in the mitochondria was observed between 0.8 and 4 μg/ml dose groups and negative control group for 24 and 48 hours exposure duration. No significant difference was observed between treated groups and negative control group for MDA test. However, there was significant difference between treated groups and negative control group for comet assay. No significant difference was observed among treated groups for different exposure time in all the assays. CONCLUSION： In the range of experimental dosage and exposure duration, ROS increase in both cytoplasmic and mitochondria and DNA damage were observed, while peroxidation of lipid was not evident.

关 键 词：烹调油烟颗粒物 氧化应激 活性氧 丙二醛 DNA断裂 

分 类 号：R114[医药卫生—卫生毒理学]