DNATyper^(TM)15试剂盒的确证试验  

Validation of the DNATyper^(TM)15 PCR amplification kit

作　　者：姜成涛[1] 叶健[1] 赵兴春[1] 季安全[1] 刘雅诚[2] 姜先华 王新淮 周怀谷 王林生[6] 吴微微[7] 牛勇 匡金枝 俞卫东[10] 

机构地区：[1]公安部物证鉴定中心,北京100038 [2]北京市公安局法医检验鉴定中心,北京100085 [3]辽宁省刑事科学技术研究所,辽宁沈阳110032 [4]公安部科技局,北京100741 [5]上海市刑事科学技术研究所,上海200083 [6]江苏省公安厅物证鉴定中心,江苏南京210024 [7]浙江省公安厅物证鉴定中心,浙江杭州310009 [8]公安部刑侦局,北京1000741 [9]天津市刑事科学技术研究所,天津300193 [10]南京市公安局刑警支队,江苏南京2100125

出　　处：《中国法医学杂志》2008年第2期73-76,81,共5页Chinese Journal of Forensic Medicine

摘　　要：目的测试DNATyperTM15试剂盒的技术性能指标,评估其法医学应用能力。方法制定测试方案,从方法学验证、灵敏度、混合样本、批次间试剂稳定性及批量样本测试、DNA提取方法适应性测试、各类常见检材的测试、稳定性测试等8个方面进行测试,并与IdentifilerTM和PowerPlex16试剂盒进行比较。结果DNATyperTM15试剂盒灵敏度较高,批次间性能稳定,对各类案件检材和DNA提取方法具有较好的适应性,具有检验混合DNA样本检测的能力。结论DNATyperTM15在上述性能指标等方面已经达到国际同类产品的技术水平,可用于法庭科学的检案与建库。Objective Developmental validation studies were designed according to the standards of forensic DNA community on DNATyper^TM15 kit ,which simultaneously amplifies 14 STR loci （D6S1043. D21 S11 .D7S820, CSF1PO. D2S1338. D3S1358, D13S317, D8S1179. D16S539. Penta E. D5 S818, VWA. D18S51 .FGA ） and amelogenin, a sex-determining locus. Methods Several key factors were tested including： Ⅰ. amount of hot-start Taq polymerase; Ⅱ. annealing temperature; Ⅲ sensitivity; Ⅳ. reaction volume; Ⅴ. cycle number; Ⅳ. primer concentration. DNATyper^TM15 was also compared with two other widely used commercial STR amplification kits, namely Identifiler^TM and PowerPlex. Results No difference in performance was observed with three lots of DNATyperTS15. Performance was not affected even after 20 times of repeated freeze-and-thaw. All three kits performed comparably in the following aspects： Ⅰ sensitivity ; Ⅱ. ability to genotype mixed samples ;Ⅲ amplification of DNA from various sample sources. DNA extraction methods （Chelex-100, magnetic beads, silica beads） did not result in any observable effect on performance with any of the three kits. Conclusion All the results demonstrated that DNATyper^TM 15 is suitable for both forensic DNA database work and casework.

关 键 词：法医物证学 DNATyper^TM15 确证试验 灵敏度 短串联重复 

分 类 号：D919[医药卫生—法医学]