家兔死后不同时间角膜基质胶原纤维的超微结构观察  

An ultrastructural observation of corneal collagen matrix of rabbit in the different time after death

作　　者：朱少华[1] 方东[1] 王继承 范氾[1] 张磊[1] 郑娜[1] 郑国翰[3] 刘良[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]驻马店市公安局刑警支队,河南驻马店463000 [3]武汉市公安局文保分局,湖北武汉430023

出　　处：《中国法医学杂志》2008年第2期99-101,146,共4页Chinese Journal of Forensic Medicine

基　　金：教育部回国留学人员科研启动基金资助项目(教外司留2003-406)

摘　　要：目的探讨角膜基质胶原纤维直径变化与死亡时间的关系。方法28只家兔空气栓塞致死后,按死后不同时间取角膜,常规方法制作超薄切片,应用透射电镜观察角膜基质0.5μm×0.5μm区域内的胶原纤维,并用图像分析系统测量反映胶原纤维直径变化的横切面面积(Y1)、周长(Y2)、平均直径(Y3)、等效直径(Y4)4个参数,所得数据用EXCEL2000和SPSS10.0软件进行统计分析。结果在死后0～72h内,角膜胶原纤维横断面的面积从1131±53nm增加到1628±26nm,周长从132.8±23nm增加到167.5nm,等效直径从38nm增加到45nm,平均直径从37.71±6nm增加到44.89±5nm。结论死后72h内,角膜基质胶原纤维直径随死后时间的延长逐渐增大,4个参数有望作为判断死后时间的新指标。Objective To study the relationship between the uhrastructural changes of corneal matrix collagen fibrils diameter and the postmortem interval. Methods Twenty-eight rabbits were killed by the method of air embolism and the samples of cornea were separately cut down in 0, 6,12,18,24, 48 and 72h after death. The uhrastructure of the collagen diameter was observed by transmission electro microscopy （TEM） and the 4 parameters of area（ Y1 ）, perimeter（Y2） , average-diameter （ Y3 ） and meandiameter（ Y4 ） were measured by a computer image technique and analyzed by Excel 2000 and SPSS 10. 0 software. Results The 4 parameters of Y1 ,Y2,Y3 and Y4 were respectively increased from 1 131 ± 53nm to 1 628± 26nm, from 132.8 ±23nm to167.5nm, from 38nm to 45nm and from 37.71 ±6nm to 44.89 ±5nm within 72 hours after the death. Conclusion The corneal matrix collagen diameter increase regularly with the postmortem interval in 72h after death, and the parameters of Y1, Y2, Y3 and Y4 could be used as a new reference mark for timing the postmortem interval after death.

关 键 词：法医病理学 角膜混浊 胶原纤维 死亡时间 透射电镜 图像分析 

分 类 号：D919[医药卫生—法医学]