利用γ-丁内酯衍生物研究空泡化与细胞分化  

The vacuolization and differentiation of vascular endothelial cells by a γ-butyrolactones derivative

作　　者：井庆川[1] 赵静[2] 赵宝祥[3] 张尚立[2] 苗俊英[2] 

机构地区：[1]山东省农业科学院家禽研究所,山东济南250023 [2]山东大学生命科学学院发育研究所,山东济南250100 [3]山东大学化学与化工学院有机化学研究所,山东济南250100

出　　处：《山东大学学报（理学版）》2008年第3期6-9,共4页Journal of Shandong University(Natural Science)

基　　金：山东省自然科学基金资助项目(Z2006D02)

摘　　要：从化学遗传学的角度,利用γ-丁内酯衍生物研究了成纤维细胞生长因子(fibroblast growth factor,FGF)诱导鸡胚盘细胞向血管内皮细胞分化过程中,空泡化对血管内皮细胞分化的影响。中性红染色及吖啶橙染色结果表明,在血管内皮细胞分化过程中出现的膜泡为酸性膜泡,γ-丁内酯衍生物可能通过抑制这些酸性膜泡,抑制了鸡胚盘细胞向血管内皮细胞的分化。该研究结果进一步证实了空泡化在细胞分化中的作用,并为研究γ-丁内酯衍生物的靶蛋白在鸡胚盘细胞向内皮细胞分化中的作用打下了基础。The effects of vacuolization on the differentiation of vascular endothelial cells were analyzed from a chemical biochemistry genetics perspective using the blastodisc cells differentiation model induced by FGF with the γ-butyrolactones derivative. The results of neutral red staining and acridine orange staining show that the vacuoles are acid, γ-butyrolactones derivative can suppress the differentiation of chick blastodisc cells to vascular endothelial cells by inhibiting the acid vacuoles in cells. The data validates the effect of vacuolizationon cell differentiation, which can be regarded as a reference for future research on the target protein functions of γ-butyrolactones derivative in chick blastodisc cells differentiation to endothelial cells.

关 键 词：鸡胚盘细胞 空泡 内皮细胞分化 化学遗传学 γ-丁内酯衍生物 

分 类 号：Q2[生物学—细胞生物学]