胰岛素样生长因子-1抗氧化损伤雪旺细胞凋亡的实验研究  

Anti-apoptosis effect of insulin-like growth factor-1 on oxidative injured Schwann cells

作　　者：丛丽[1] 李振华[1] 孙晋浩[1] 暴丽华[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《山东大学学报（医学版）》2008年第3期221-224,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(Y2007C031);山东省优秀中青年科学家科研奖励基金(Y2007C003)

摘　　要：目的探讨胰岛素样生长因子-1(IGF-1)对H2O2损伤的雪旺细胞(SCs)抗凋亡的作用。方法将培养的SCs分为H2O2处理组、H2O2加IGF-1保护组和正常对照组。检测各组细胞内超氧化物歧化酶(SOD)及丙二醛(MDA)的含量,丫叮橙(AO)染色观察细胞存活、凋亡情况,蛋白印迹(Western blot)法检测Bcl-2的表达水平。结果与正常组及保护组相比,H2O2处理组细胞具有典型的凋亡形态特征:核染色质浓染、边集,细胞体积缩小,细胞存活率降低;SOD含量明显减少(P<0.01),MDA含量明显增加(P<0.01),Bcl-2表达下调;而IGF-1保护组细胞存活率明显升高(P<0.01),SOD含量较损伤组增高(P<0.01),MDA含量较损伤组明显减少(P<0.01),Bcl-2表达明显上调。结论本实验结果提示IGF-1对氧化损伤的SCs具有保护作用。Objective To investigate the anti-apoptosis effects of insulin-like growth factor-1 on oxidative injured Schwann cells （SCs）. Methods Cultured SCs of rats were divided into the H2O2 group, the H2O2 and IGF-1 group, and the control group. The amounts of intracellular superoxide dismutase （hepatocuprein, SOD） and malondialdehyde（malonaldehyde, MDA）were determined by a biological chemical method, apoptosis was determined by acridine orange staining, and Bcl-2 expression was determined by Western blot. Results Activities of SCs in the oxidative injured group decreased compared with the control group. However, these changes were significantly reversed by the IGF-1 pre-conditioning. Conclusion IGF-1 has an anti-apoptosis effect on oxidative injured SCs.

关 键 词：胰岛素样生长因子-1 雪旺细胞 细胞凋亡 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]