大鼠动脉粥样硬化形成中凝血酶活化的纤溶抑制剂与血脂及凝血的关系研究  

Correlation between TAFI and blood fat and coagulation in the process of atherosclerosis

作　　者：刘利妍[1] 刘向群[1] 陈焕芹[1] 陈效安[1] 

机构地区：[1]山东大学齐鲁医院保健科,山东济南250012

出　　处：《中国病理生理杂志》2008年第4期712-715,共4页Chinese Journal of Pathophysiology

基　　金：山东省科技攻关计划资助项目(No2006GG2202045)

摘　　要：目的:探讨凝血酶活化的纤溶抑制剂(TAFI)活性在大鼠动脉粥样硬化(AS)形成过程中的作用机制。方法:将40只健康雄性Wistar大鼠随机分为4组(每组10只),包括正常对照组、高脂组、高脂维生素D3组和高脂维生素D3+内皮损伤组。分别以普通饲料、高脂饲料、高脂饲料+维生素D3、高脂饲料+维生素D3+内皮球囊损伤处理,复制大鼠动脉粥样硬化形成过程中的3个阶段的模型,即高脂血症、纤维增生性动脉硬化、较成熟的AS斑块病变。用全自动生化仪测定血脂系列,全自动血凝仪测定凝血系列,发色底物法测定血浆TAFI的活性。结果:3组模型鼠的血浆总胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)、纤维蛋白原(Fib)水平和TAFI活性明显高于正常对照组(P<0.01),有递增趋势,以高脂维生素D3+内膜损伤组最为明显;3组模型的血浆高密度脂蛋白(HDL-C)、凝血时间(PT)、白陶土部分凝血活酶时间(APTT)水平明显低于正常对照组(P<0.01或P<0.05),以高脂维生素D3+内膜损伤组最为明显。血浆TAFI活性与TC、TG和Fib水平呈正相关。结论:动脉粥样硬化过程中TAFI水平升高,血浆TAFI水平与血脂及凝血相关,提示TAFI可能参与AS的形成过程,TAFI活性升高可能是动脉粥样硬化的致病因素之一。AIM. To investigate the relationship between the thrombin -activatable fibrinolysis inhibitor （TAFI） level in plasma and atherosclerosis, the TAFI level in plasma and blood fat and blood clotting index. METHODS： Totally 40 healthy male Wistar rats were randomly divided into 4 groups （n = 10）, control group, high lipid group, high lipid ＋ vitamin D3 overload group, and high lipid ＋ vitamin D3 ＋ endothelium injure group by treating the animals with normal diet, high lipid, high lipid ＋ vitamin D overload, and high lipid ＋ vitamin D overload ＋ endothelium injury, respectively, for inducing three stages of AS in rats. Then, the total cholesterol （ TC ）, total triglyeride （TG）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, and prothrombin time （PT）, activated partial thromboplasin time （ APTT）, fibrinogen （ Fib）, the activity of TAFI were measured. RESULTS： TC, TG, LDL - C, Fib and the activity level of TAFI in plasma in three model groups increased gradually compared with the control （P 〈 0.01 ）. HDL- C, PT and APTT in plasma of three model groups decreased gradually compared with the control （ P 〈 0.01 or P 〈 0. 05 ）. The activity of TAFI in plasma had positive correlation with TG, TC and Fib. CONCLUSION： The activity of TAFI increases in the progress of atherosclerosis. The activity of TAFI in plasma has a correlation with blood fat and coagulation, which indicates that TAFI might participate in the process of atherosclerosis, and TAFI is one of etiological factors of atherosclerosis.

关 键 词：动脉硬化 凝血酶活化纤维蛋白溶解抑制剂 内皮损伤 

分 类 号：R541.4[医药卫生—心血管疾病]