急性重复低氧对小鼠脑组织血氧饱和度、线粒体功能以及ATP水平的影响  

Effects of repeated hypoxic exposures on saturation oxygen,mitochondrial function and ATP levels in the mouse brain

作　　者：谢胜男[1] 李昕[1] 李尧华[1] 刘耀波[2] 于顺[1] 

机构地区：[1]首都医科大学宣武医院老年病研究所神经生物学研究室 [2]首都医科大学宣武医院低氧医学研究所,北京100053

出　　处：《中国病理生理杂志》2008年第4期755-758,共4页Chinese Journal of Pathophysiology

基　　金：北京市自然科学基金重大项目(No7050001)

摘　　要：目的:探讨急性重复低氧对脑组织血氧饱和度与线粒体功能的影响。方法:将BALB/c小鼠置于低氧密闭罐中,通过小鼠呼吸消耗罐内氧造成罐内低氧,以小鼠出现喘呼吸为低氧耐受极限,然后将小鼠转到另一低氧密闭罐中,依此类推,连续进行5次低氧。记录小鼠每次的缺氧耐受时间、局部脑组织血氧饱和度,测定每次低氧暴露结束时的脑组织的线粒体复合体I活性和ATP含量。结果:小鼠的缺氧耐受时间随缺氧暴露次数增加而显著延长。脑组织血氧饱和度在第1、2次缺氧暴露时急剧下降,但在第3、4、5低氧暴露时先小幅度下降再逐渐恢复至正常水平,然后再缓慢降低。脑组织线粒体复合体I的活性随着缺氧次数的增加逐渐被抑制,ATP含量在第1次低氧暴露结束时低于正常水平,在第3、5次低氧暴露结束时高于正常水平。结论:急性重复低氧导致脑组织血氧饱和度降低的速度减慢、线粒体功能抑制以及脑组织ATP水平增高,后者很可能是动物脑组织耐缺氧能力增强的重要机制。AIM： To study the effects of repeated ,hypoxic exposures on saturation oxygen and mitochondrial function in the brain. METHODS ： A BALB/C mouse was placed in an air - sealed jar and hypoxic environment in the jar was established through consumption of the oxygen by respiration of the mouse. A gasp breath was regarded as the hypoxic tolerant limit of the mouse. The mouse was then transferred to a new jar. The mouse was exposed to hypoxia in this way for 5 times. In each run of hypoxic exposure, the hypoxic tolerance time, local brain saturation oxygen was measured. By the end of each hypoxic exposure, the mitochondrial complex I activity and ATP levels in the brain were also examined. RESULTS： The hypoxic tolerant time of a mouse increased during repeated hypoxic exposures. The saturation oxygen in the brain declined quickly during the first two hypoxic exposures, but recovered to the normal levels after a short fall during the third to fifth hypoxic exposures. The mitochondrial complex I activity decreased during repeated hypoxic exposures. The ATP levels declined by the end of the first hypoxic exposure, but increased by the end of the third and fifth hypoxic exposure. CONCLUSION： Repeated hypoxic exposures lead to increased oxygen saturation and ATP levels but decreased mitochondrial function. The increased ATP levels may be responsible for the increased hypoxic tolerance of the mouse brain.

关 键 词：低氧 小鼠 脑 线粒体 氧饱和度 

分 类 号：R338[医药卫生—人体生理学]