乳块消片高效液相指纹图谱研究  

Study on HPLC Fingerprinting of Rukuaixiao Tablets

作　　者：展晓日[1] 史新元[1] 张培[1] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2008年第4期1-3,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市科技计划课题资助课题(D02050040040111);北京市"中药基础与新药研究重点实验室"资助课题

摘　　要：目的:建立乳块消片的高效液相色谱指纹图谱,为科学客观地评价其质量提供可靠的方法。方法:利用HPLC-DAD方法,梯度洗脱,测定了12批乳块消片的指纹图谱。色谱条件为:Eclipse XDB-C18柱(4.6 mm×250 mm,5μm);流动相:采用甲醇(A)-3%醋酸水(B)体系;流速:1 mL.min-1;柱温:30℃;UV检测波长:280 nm。结果:12批乳块消片得到的色谱指纹图谱有28个共有峰,通过与对照品的保留时间及紫外光谱的比对,确定3,8,25,27号峰分别为丹参素、原儿茶醛、橙皮苷、丹酚酸B。结论:乳块消片的指纹图谱特征性及专属性强,可结合含量测定用于全面控制乳块消片的质量。Objective：To develop a sensitive and specific HPLC method for controlling the quality of Rukuaixiao tablets. Methods：The fingerprinting analysis of Rukuaixiao tablets was carried out on an Eclipse XDB-C18 column （4.6 mm × 250 mm, 5 μm）with methanol and containing water solution as mobile phases in a gradient mode. The flow rate was 1 mL·min^-1, the column temperature was 30 ℃, and the detection wavelength was 280 nm. Result： 28 characteristic peaks were found among 12 batches of samples and four peaks of them identified by being compared with the retention time and the UV spectra of reference substances. Conclusion： The method is accurate and reliable with high specificity and can be used for the quality control of Rukuaixiao tablets.

关 键 词：乳块消片 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]