人参二醇组皂苷对心肌梗死犬血清一氧化氮、一氧化氮合酶水平的影响  

Effect of Panaxadiol Saponins on NO,NOS Level in Serum in Acute Myocardial Infarction Dogs

作　　者：刘洁[1] 刘芬[1] 王秋静[1] 赵雪俭[1] 

机构地区：[1]吉林大学白求恩医学院,吉林长春130021

出　　处：《中国实验方剂学杂志》2008年第4期46-49,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技厅自然科学基金资助项目(20030501)

摘　　要：目的:观察人参二醇组皂苷(PDS)对急性心肌梗死(AMI)犬血清一氧化氮(NO)、一氧化氮合酶(NOS)水平及心肌超微结构的影响。方法:结扎犬左冠状动脉前降支(LAD)建立AMI模型。采用分光光度法检测血清NO、NOS水平;定量组织学硝基四氮唑兰(N-BT)染色法,测定心肌梗死面积(MIS)以及应用透射电子显微镜观察心肌细胞超微结构的改变。结果:与模型组比较,PDS能明显提高AMI犬血清NO含量和NOS活性;减少MIS、减轻心肌缺血引起的心肌细胞超微结构的损伤。结论:PDS对AMI犬心肌细胞有保护作用,可能与其提高血清NO和NOS水平有关。Objective：To investigate the effects of Panaxadiol Saponins （PDS） on the nitric oxide（NO） and nitric oxide synthase（NOS） in serum in acute myocardial infarction （AMI） dogs. Methods： An AMI model was made by ligating left coronary artery in dogs. The content of NO and activity of NOS in serum were detected by spectrophotometric method. N-BT staining method was used to delermin the extent of myocardial infarction and the change in myocardial ultrastructure was observed by transmission electron microscope. Results ： Compared with model group, PDS increase the content of NO and activity of NOS in serum, and decrease the extent of myocardial infarction significantly. PDS can alleviate the change induced by ischemia in myocardial ultrastructure markedly. Conclusions： PDS has apparently protective effects on myocardium in acute myocardial infarction dogs. Its mechanisms probably liein increasing NO and NOS level in serum.

关 键 词：人参二醇组皂苷 心肌梗死 一氧化氮 一氧化氮合酶 心肌/超微结构 

分 类 号：R285.5[医药卫生—中药学]