清毒片对HL-60细胞尿激酶型纤溶酶原激活剂活性的体外影响  

The Influence of Qingdupian on the Expression of u-PA in HL-60 Cells

作　　者：王文花[1] 杨洪涌[2] 刘安平[2] 陈鹏[2] 赵珍品[2] 

机构地区：[1]温州医学院附属第二医院中医教研室,浙江温州325003 [2]广州中医药大学附属第一医院血液科,广东广州510405

出　　处：《中国实验方剂学杂志》2008年第4期60-63,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察中药清毒片对HL-60细胞尿激酶型纤溶酶原活化剂(u-PA)活性的影响。方法:取离心灭菌后的清毒片药物水煎浓缩剂2 mL,以含10%胎牛血清的RPMI-1640培养液逐步稀释为从1∶10至1∶1 600的12个稀释倍数,分别培养等量细胞,同时以阿糖胞苷作为实验的阳性对照,以培养液作为阴性对照。用MTT法分别测定各浓度药液的细胞增殖抑制率,取上清进行u-PA活性的ELISA检测。结果:清毒片水煎浓缩剂对HL-60细胞有较好的抑制作用,随着药物浓度加大,抑制作用增强,同时细胞上清中的u-PA活性下降。结论:清毒片对急性早幼粒细胞性白血病的治疗作用与其降低白血病细胞u-PA活性水平的作用有关。Objective：To observe the effects of Qingdupian on the u-PA expression in HL-60 cells. Methods： 2 mL of Qingdupian was diluted with RPMI-1640 culture medium supplemented with 10% fetal bovine serum to 12 diluted liquids from 1 - 10 to 1 ： 1 600 concentrations. Ara-c was used as the positive control, and the culture medium as the negative control. MTT assay was performed. The supernatant was collected for ELISA essay to determine the activity of u- PA. Results： The results research showed that Qingdupian fluid has inhibiting effects on HL-60 cells. And the u-PA activity in the supernatant fluid dropped with the raise of medicine concentration. Conclusions： It suggests that the curative effect of Qingdupian is related to its effect of declining u-PA activity on leukemia cells.

关 键 词：清毒片 HL-60细胞 尿激酶型纤溶酶原激活剂 

分 类 号：R285.5[医药卫生—中药学]