Matrigel对羊水间充质干细胞成组织能力的影响  

Effects of Matrigel on the Morphogenesis of Amniotic Fluid-derived Mesenchymal Stem Cells in Vivo

作　　者：杜玉峰[1] 耿红全[1] 潘骏[2] 朱哲[1] 齐隽[1] 康健[1] 谢华[1] 陈方[1] 

机构地区：[1]上海交通大学医学院附属新华医院泌尿外科,上海200092 [2]上海市儿科医学研究所,上海200092

出　　处：《航天医学与医学工程》2008年第2期112-116,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(30271351)

摘　　要：目的研究Matrigel对羊水间充质干细胞在裸鼠皮下成组织能力的影响。方法将羊水间充质干细胞与Matrigel混合,注入裸鼠皮下,14d后观察新生物的形态外观和组织结构。并以不混合Matrigel的羊水间充质干细胞注入裸鼠皮下作为阴性对照,单纯Matrigel注入裸鼠皮下作为空白对照。结果实验组和阴性对照组在裸鼠皮下均形成一长圆形瘤样组织,其形态外观基本一致,但组织结构有明显差异,在实验组组织血供丰富,主要为腺样结构,部分含管腔,向平滑肌细胞,上皮细胞和幼稚神经细胞分化。在阴性对照组血供较少,细胞呈单一平铺排列,未观察到明显组织结构,细胞未分化。空白对照组无新生组织形成。结论Matrigel能促使羊水间充质干细胞在裸鼠皮下向成熟的组织细胞分化并形成简单的腺样组织结构。Objective To investigate the effect of Matrigel on the morphogenesis of amniotic fluid-derived mesenchymal stem cells in vivo. Methods Amniotic fluid-derived mesenchymal stem cells （MSC） were mixed with Matrigel, injected into nude mice. 14 d later, new subcutaneous tissues were harvested. Gross appearances and tissue structures were observed. Negative and blank controls were set up by non-Matrigel mixed amniotic fluid-derived MSCs and Matrigel alone injected, respectively. Results Both the experimental group and the negative control group had subcutaneous tissues .generated, though the gross appearances were similar, which were ellipse-shaped tumor like tissues ,but tissue structures were obvious different. In the experimental group, blood supplies were abundant,and gland-like structures were observed, some of which contained a lumen. There were differentiated smooth muscle cells, epithelial cells and early nerve cells distributed in tissues. In the negative control group, blood supplies were relatively rare, cells were simply tiled and remained undifferentiated, no special structures were observed. Matrigel was absorbed in blank control group. Conclusion Matrigel can promote the differentiation of amniotic fluid-derived MSCs and form simple gland-like structures.

关 键 词：羊水间充质干细胞 组织工程 MATRIGEL 细胞外基质 

分 类 号：Q813[生物学—生物工程]