保肝宁对肝纤维化模型大鼠肝组织瘦素受体及其JAK2/STAT3信号通路的影响  

Effect of Baogan Ning on Hepatic Leptin Receptor and Its JAK2/STAT3 Message Pathway in Rats with Hepatic Fibrosis

作　　者：贺松其[1] 文彬[2] 吕志平[1] 侯丽颖[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]解放军四五八医院,广东广州510602

出　　处：《广州中医药大学学报》2008年第1期60-62,67,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(编号:30672769);广东省中医药局资助课题(编号:1050128)

摘　　要：【目的】探讨保肝宁抗肝纤维化的作用机制。【方法】将50只Wistar大鼠随机分为5组:正常组,模型组,保肝宁高、低剂量组(剂量分别为35.4、17.7 g/kg),秋水仙碱组(剂量为0.11 mg/kg);采用复合因素复制肝纤维化大鼠模型,提取肝组织蛋白,采用Western Blot法检测肝组织瘦素受体(OB-Rb)、JAK2、STAT3表达情况。【结果】正常组蛋白表达量最少,各药物处理组蛋白条带颜色均较浅,模型组条带颜色最深。与正常组比较,模型组OB-Rb、JAK2、STAT3蛋白表达程度均增高;与模型组比较,各药物处理组的OB-Rb、JAK2、STAT3蛋白表达明显降低;保肝宁高、低剂量组与秋水仙碱组比较,OB-Rb、JAK2、STAT3蛋白表达明显降低。【结论】保肝宁抗肝纤维化的作用可能与其能抑制OB-Rb表达,进而抑制JAK2/STAT3信号通路有关。Objective To explore the anti-hepatic-fibrosis mechanism of Baogan Ning（BN）.Methods Fifty Wistar rats were randomized into 5 groups： normal group,model group,high-and low-dosage BN groups（35.4 and 17.7 g/kg respectively）,and colchicine group（0.11mg/kg）.Rat models of hepatic fibrosis were induced by multiplex factors.Hepatic protein was extracted,and the expression of hepatic leptic receptors of OB-Rb,JAK2 and STAT3 was detected with Western Blot method.Results The protein expression was not obvious in the normal group,and the color of protein band in the medication groups was light but dark in the model group.Compared with the normal group,the expression of OB-Rb,JAK2 and STAT3 increased in the model group.However,the expression of OB-Rb,JAK2 and STAT3 decreased in the medication groups as compared with the model group.And the expression of OB-Rb,JAK2 and STAT3 decreased in the BN groups as compared with the colchicines group.Conclusion The anti-hepatic-fibrosis mechanism of Baogan Ning is probably related with the inhibition of OB-Rb expression,thus inhibit the JAK2/STAT3 message pathway.

关 键 词：保肝宁/药理学 肝纤维化/中药疗法 蛋白表达 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]