蚕豆根尖微核试验在辣子草化感作用研究中的应用  

Assessment of alleopathy of Galinsoga parviflora Cav. using Vicia-micronucleus test

作　　者：范雪涛[1,2] 马丹炜[1,2] 刘爽[1,2] 向莎[1,2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610066 [2]四川师范大学细胞生物学研究室,四川成都610066

出　　处：《生态环境》2008年第1期323-326,共4页Ecology and Environmnet

基　　金：四川师范大学校级重点项目(061k008)

摘　　要：运用蚕豆(Vicia faba L.)根尖细胞微核试验,研究入侵植物辣子草(Galinsoga parviflora Cav.)水浸提液的化感效应,结果表明,辣子草水浸提液具有一定的遗传毒性。在辣子草水浸提液作用下,蚕豆根尖细胞有丝分裂各时相受到影响,有丝分裂指数下降,微核率升高,细胞中出现了微核、染色体断片、染色体桥、染色体滞留等多种染色体畸变,辣子草水浸提液对蚕豆根尖的影响存在显著的时间效应和浓度效应。蚕豆根尖微核技术能较好的反映辣子草化感作用潜力,在评估入侵植物的化感作用方面具有较大的应用价值。The aUeopathic effects of aqueous extracts of Galinsoga parviflora Cav. were studied by using Vicia-micronucleus test. The results showed that the aqueous extracts of G parviflora had genetic toxicity on Viciafaba root tip cells. The aqueous extracts of G parviflora disturbed mitosis, the mitotic index decreased, the micronucleus frequency increased, and aberrant chromosomes such as micronucleus, chromosome break, chromosome bridge and chromosome lag occurred. These effects were concentration dependent and time dependent. Vicia-micronucleus test could assess the allelopathy of G parviflora and maybe is a promising technique in assessment of the allelopathy of invasive plants.

关 键 词：入侵植物 辣子草 化感作用 蚕豆根尖细胞微核试验 遗传毒性 

分 类 号：Q945[生物学—植物学] X835[环境科学与工程—环境工程]