小檗碱、绿原酸和黄芩苷对LPS损伤大鼠肠黏膜微血管内皮细胞表达ICAM-1的影响  

Effects of Berberine,Chlorogenic Acid and Baicalin on the Expression of ICAM-1 in Rat Intestinal Mucosa Microvascular Endothelial Cells Injured by LPS

作　　者：张涛[1] 黄会岭[2] 胡格[3] 段慧琴[3] 滕可导[1] 穆祥[3] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]河北农业大学动物科技学院,保定071001 [3]兽医学(中医药)北京市重点实验室,北京102206

出　　处：《畜牧兽医学报》2008年第4期499-502,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30500368);北京市自然科学基金(6021001);北京市科技新星项目(2006A26)资助

摘　　要：为研究中药有效成分小檗碱、绿原酸和黄芩苷的抗炎机制,通过体外培养大鼠肠黏膜微血管内皮细胞(Rat intestinal mucosa microvascular endothelial cells,RIMMVECs)建立LPS损伤模型,采用免疫细胞化学方法观察了3种中药成分对LPS损伤RIMMVECs表达ICAM-1的影响,并对染色结果进行了图像灰度分析与统计学检验。研究发现,RIMVECs正常状态下低水平表达ICAM-1,用LPS刺激后ICAM-1的表达明显升高,而中药有效成分小檗碱、绿原酸和黄芩苷能够抑制LPS诱导的RIMVECs强阳性表达ICAM-1。结果表明,小檗碱、绿原酸和黄芩苷的抗炎作用与其下调ICAM-1的表达密切相关,为阐明其抗炎机制提供了数据。To investigate their anti-inflammatory mechanism, the effects of berberine （Ber.）, chlorogenic acid （Ch. A. ） and baicalin （Bai） on the expression of intercellular adhesion molecule 1 （ICAM-1） in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） challeged by lipopolysaccharide （LPS） were analyzed by immunocytochemical staining, and gray scale and statistical analysis were performed. The results showed that RIMMVECs constitutively express ICAM-1 in low level and LPS significantly increased it, while LPS-induced strong expression of ICAM-1 in RIMMVECs was suppressed by Ber, Ch. A and Bai. The results indicated that the anti-inflammatory role of Ber, Ch. A and Bai is related to their downregulating effect on LPS-mediated ICAM-1 expression, which provide data for clarifying their mechanism.

关 键 词：小檗碱 绿原酸 黄芩苷 细菌脂多糖 微血管内皮细胞 

分 类 号：S853.74[农业科学—临床兽医学]