不同品种绵羊TGF-β1基因单核苷酸多态性及其与繁殖力关系的研究  

The Analysis for the Single Nucleotide Polymorphism of TGF-β1 and Its Association with Reproduction in Different Breeds Sheep

作　　者：高丽霞[1] 李宏滨[1] 杜立新[1] 宋雪梅[1] 李珍祖[2] 李善刚[1] 魏彩虹[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所国家畜禽分子遗传育种中心,北京100094 [2]山东师范大学生命科学学院,济南250014

出　　处：《畜牧兽医学报》2008年第4期503-507,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30270949);“863”计划项目(2006AA10Z199)

摘　　要：以小尾寒羊、滩羊、同羊、欧拉羊共计196头为研究材料,采用PCR-SSCP技术,对绵羊TGF-β1基因6-7外显子区间内的805 bp序列进行多态性分析,发现了一个多态位点。经克隆测序分析发现,第6内含子区内存在一个突变,该突变位点为第7外显子上游的第294位碱基处缺失了AGAC(序列:gi76871756中的14201位)。对不同绵羊群的基因型和等位基因频率统计结果表明,多胎品种小尾寒羊以AB基因型为主。χ2检验结果表明,单胎品种滩羊、同羊、欧拉羊以BB基因型为主,所有品种都处于Hardy-Weinberg平衡状态。A single nucleotide polymorphism （SNP） of sheep 805 bp sequence in 6th intron region of transforming growth factor/31 （TGF-β1） gene was identified in 196 sheep among Small-tailed Han,Tong,Tan and Oula sheep by PCR-SSCP, which declared one mutation point. Comparative sequencing analysis of cloned products revealed a AGAC deletion at upstream 294 of extron 7 of TGF-β1 gene （site 14 201 in gi76871756）. The statistical results of genotype and allele frequency in different breeds showed that the genotype AB dominated in the Small-tailed Han sheep. The genotype BB was in majority in low-fecudity sheep. The results of x^2 test indicated that all the populations were in Hardy-Weinberg equilibrium.

关 键 词：转化生长因子Β1 PCR—SSCP 单核苷酸多态性 绵羊 

分 类 号：S826.2[农业科学—畜牧学]