内蒙古绒山羊CDK2基因的克隆与序列分析  

Cloning and Characterization of the CDK2 Gene cDNA of Inner Mongolia Cashmere Goats

作　　者：关泽红[1] 旭日干[1] 赵燕芳[1] 毕力格[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特010021

出　　处：《畜牧兽医学报》2008年第4期508-512,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划项目(2002AA242061);国家自然科学基金(3060009)资助项目

摘　　要：采用同源序列克隆技术结合RT-PCR和RACE技术,首次从内蒙古绒山羊睾丸组织中克隆出羊CDK2基因的全长cDNA序列(GenBank登录号为EF035041)。结果显示:羊CDK2基因的cDNA序列长为1 355 bp,5′端非翻译区为174 bp,3′端非翻译区为266 bp,开放阅读框为894 bp,编码298个氨基酸。与牛CDK2基因cDNA序列同源性为98%,氨基酸序列完全一致;和其他哺乳动物CDK2基因的cDNA序列同源性也达92%以上;氨基酸序列同源性为93%以上。说明CDK2在结构和功能上有很高的保守性。The complete CDK2 cDNA sequence from Inner Mongolia Cashmere goat testis was cloned in the first time by homology cloning approach combined with RT-PCR and RACE technique （ GenBank Accession No. EF035041）. The CDK2 cDNA sequence was 1 355 bp length, containing an open reading frame of 894 bp, flanked by 174 bp 5′UTR and 266 bp 3′ UTR, encoding 298 amino acids. The homology of the goat CDK2 cDNA sequence with bovine was 98%. The amino acids sequence of the CDK2 protein shared 100% identity with bovine CDK2 protein. The homology of the Goat CDK2 cDNA sequence and amino acids sequence with the other mammalian CDK2 genes was more than 92% and 93%, respectively, showing that the structure and function of CDK2 gene is highly conservative.

关 键 词：内蒙古绒山羊 CDK2基因 克隆 RACE 

分 类 号：S827.2[农业科学—畜牧学]