调中颗粒对大鼠混合反流性食管炎的治疗作用及其机制  

Therapeutic effects of Tiaozhong Granule on esophagus mucous membrane in rats with mixed reflux esophagitis and the mechanisms

作　　者：时昭红[1] 苏文[1] 张介眉[1] 郝建军[1] 冯云霞[1] 陈洲[2] 

机构地区：[1]武汉市第一医院消化内科,湖北武汉430022 [2]湖北中医学院中医系,湖北武汉430065

出　　处：《中西医结合学报》2008年第4期366-371,共6页Journal of Chinese Integrative Medicine

基　　金：武汉市卫生局临床重点学科研究项目[No.武卫(2003)228号]

摘　　要：目的:观察调中颗粒对混合反流性食管炎大鼠食管黏膜病理改变及血清氧自由基的影响,探讨调中颗粒治疗反流性食管炎的作用机制。方法:58只SD大鼠随机分成5组:调中颗粒组、半夏泻心汤组、西沙必利组、模型组和假手术组。通过食管十二指肠端侧吻合术法复制混合反流性食管炎大鼠模型,造模结束后灌胃给药,连续给药12d后处死大鼠,HE染色法观察病理变化,免疫组织化学SABC法检测大鼠食管黏膜中肿瘤抑制蛋白质p53和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达,比色法检测血清中丙二醛(malondialdehyde, MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)的活性。结果:治疗结束后,与模型组比较,调中颗粒组、半夏泻心汤组和西沙必利组大鼠食管黏膜组织病理改变有不同程度的修复。与模型组比较各治疗组血清MDA含量下降(P<0.05,P<0.01),SOD和GSH-Px活性升高(P<0.05,P<0.01),p53和PCNA表达减少(P<0.01)。调中颗粒在降低MDA含量、提高SOD和GSH-Px活性方面优于西沙必利片(P<0.05),减少PCNA和p53表达方面优于半夏泻心汤和西沙必利片(P<0.05)。结论:调中颗粒可以降低MDA的含量,提高SOD和GSH-Px的活性,减少PCNA和p53的表达,促进黏膜的修复,可能为其治疗反流性食管炎的作用机制。Objective： To explore the mechanism of Tiaozhong Granule （TZG）, a compound traditional Chinese herba medicine, in treating rats with mixed reflux esophagitis. Methods： Fifty-eight SD rats were randomly divided into untreated group （n = 12）. sham-operated group （n= 10）. TZG-treated group （n = 12）, Banxia Xiexin Decoction （BXXXD）-treated group （ n = 12） and cisapride-treated group （n=12）. Mixed reflux esophagitis was induced by esophago-duodenum end-to-side anastomosis. Four weeks later, the rats were orally administered twice daily for 12 days. Pathological changes of esophagus mucous membrane were observed by using HE staining. The expressions of proliferating cell nuclear antigen （PCNA） and p53 in the esophagus tissue were detected by immunohistochemical SABC method. Spectrophotometric method was used to detect the content of malondialdehyde （MDA） and the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH- Px） in serum. Results： Compared with the untreated group, pathological changes of esophagus mucous membrane were relieved in different degrees in TZG-treated group, BXXXD-treated group and cisapride-treated group. Content of MDA and expressions of PCNA and p53 were obviously decreased in the three treated groups （P〈0. 01）, and the activities of SOD and GSH-Px were significantly increased in the three treated groups （P〈 0.05, P〈0.01）. TZG had better effects than cisapride in decreasing the content of MDA and increasing the activities of SOD and GSH-Px （P〈0. 05）. TZG was better in aspect of reducing the expressions of PCNA and p53 than BXXXD and cisapride tablets （P〈0.05）. Conclusion： Tiaozhong Granule can treat mixed reflux esophagitis in rats, and its action mechanisms may be associated with decreasing the expressions of PCNA and p53 in esophagus mucous membrane, reducing the content of MDA and increasing the activities of SOD and GSH-Px in serum.

关 键 词：食管炎 反流性 复方 氧自由基 增殖细胞核抗原 肿瘤抑制蛋白质P53 大鼠 

分 类 号：R285.5[医药卫生—中药学]