玄参和附子抗鼠心肌肥厚作用的对比实验研究  

Comparative study of Scrophulariae and Aconite in inhibiting myocardial hypertrophy in rats and mice

作　　者：顾伟梁[1] 陈长勋[1] 王樱[1] 

机构地区：[1]上海中医药大学药理学教研室,上海201203

出　　处：《中西医结合学报》2008年第4期376-380,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.30572379)

摘　　要：目的:探讨寒性药玄参和热性药附子对大、小鼠心肌肥厚及神经内分泌因子的影响。方法:采用注射异丙肾上腺素及L-甲状腺素两种方法分别制备小鼠和大鼠心肌肥厚模型。随机分为5组:正常组、模型组、美托洛尔组(或卡托普利组)、玄参组和附子组。灌胃给药干预7～9d,称体质量后取血和心脏备用。称心脏质量测定左心室及全心质量指数;放射免疫法测定血浆环磷酸腺苷(cyclic adenosine mono- phosphate,cAMP)和心肌组织血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)的水平:病理切片HE染色观察心肌细胞横断面面积大小。结果:寒性药玄参能显著改善心脏质量指数,降低cAMP、AngⅡ的含量,明显缩小心肌细胞横断面面积。而热性药附子则无此作用。结论:寒性药玄参能通过抑制心肌肥厚大鼠交感神经系统活性,降低AngⅡ水平而抑制心肌肥厚。而热性药附子抗心肌肥厚及抑制神经内分泌因子分泌的作用不显著。Objective： To explore the effects of Scrophulariae of cold nature and Aconite of hot nature on myocardial hypertrophy and neuroendocrine factors in rats and mice. Methods： A mouse model of myocardial hypertrophy was established by hypodermic injection of isoproterenol. Sixty myocardial hypertrophy mice were randomly divided into five groups, normal control group, untreated group, metoprolol-treated group, Scrophulariae-treated group and Aconite-treated group. A rat model of myocardial hypertrophy was established by peritoneal injection of L-thyroxin. Fifty rats were randomly divided into five groups, normal control group, untreated group, captopril-treated group, Scrophulariae-treated group and Aconite-treated group. After 7-9 days of treatment with intragastric administration of the corre- sponding drugs, the effects of Scrophulariae and Aconite on left ventricular weight index （LVWI） and heart weight index （HWI） were determined. The concentrations of cyclic adenosine monophosphate （cAMP） in plasma and angiotensin Ⅱ （Ang Ⅱ ） in myocardium were detected through radio-immunity method. Cardiocyte cross-section area was determined by using HE staining. Results： Scrophulariae of cold nature could significantly reduce the LVWI, HWI and cardiocyte cross-section area, and could decrease the content of cAMP and Ang Ⅱ . However, Aconite had no such effects. Conclusion： Scrophulariae of cold nature can inhibit myocardial hypertrophy through restraining the activity ofsympathetic nervous system and decreasing the level of Ang Ⅱ. The inhibition of Aconite of hot nature on cardiac hypertrophy is not significant.

关 键 词：玄参 附子 心肌病 肥厚性 对比研究 大鼠 小鼠 

分 类 号：R285.5[医药卫生—中药学]