WFS1在大鼠胰腺不同发育阶段的表达  

Expression of WFS 1 in Different Development Stages of Rat Pancreas

作　　者：石慧[1] 曹丽华[1] 袁栎[1] 刘莉洁[1] 侯良芹[1] 佟辉[1] 王宁[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学与分子生物学系,南京210029

出　　处：《现代生物医学进展》2008年第4期605-607,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30670781);江苏省高校自然科学基金资助(03KJR18002)

摘　　要：目的:研究WFS1在胰腺发育不同阶段的表达和细胞定位。方法:运用Western Blot技术检测WFS1在大鼠胰腺发育不同阶段的蛋白表达水平;运用免疫荧光检测不同时期WFSl在胰腺的定位。结果:WestemBlot结果显示WFS1的蛋白表达量胚胎后期高于新生期,成年期表达量上升;免疫荧光结果显示在不同发育时期WFS1与胰岛β细胞共表达。结论:WFS1在胚胎发育中后期的高表达可能与胰岛形成及功能完善有关,并且可能参与了胰岛重塑。To investigate the expression and cell location of WFS1 during pancreas development. Methods： The expression of in different development stages of rat pancreas was examined by Western Blot; WFS 1 location in pancreas was examined by double fluorescence immunohistochemistry. Results： The expression of WFS 1 was found much higher in the anaphase of embryo than in the newborn and was rising at the adult stage. WFS 1 and pancreatic islets cell β coexisted and were expressed in the different development stages of rat pancreas. Conclusion：The expression of WFS 1 was higher in the anaphase of embryo, which may be related to formation and func- tional improvement of pancreatic islets as well as remodeling of pancreatic islets.

关 键 词：WFS1 WESTERN BLOT 免疫荧光 胰腺发育 胰岛重塑 

分 类 号：Q75[生物学—分子生物学] Q813