自然流产孕妇外周血淋巴细胞染色体着丝粒点变异与乙肝病毒感染的关系研究  

The study of relationship between aberration of chromosomal centromeric dots and infection of hepatitis B in the peripheral blood of women with spontaneous abortion

作　　者：刘彦慧[1] 何永明[1] 宋娟[1] 王明波[1] 黎丽芬[1] 石静[1] 

机构地区：[1]中山大学附属东华医院医学遗传研究室,广东东莞523110

出　　处：《医学信息（手术学分册）》2008年第2期134-136,共3页Medical Information Operations Sciences Fascicule

基　　金：广东省东莞市科技局医学科研项目20060315

摘　　要：目的研究乙肝病毒感染与外周血淋巴细胞染色体着丝粒点变异的关系,探讨孕妇自然流产原因。方法选择具有自然流产史孕妇50例,取静脉血采用ELLASA法检测乙型肝炎表面抗原,阳性者检测血转氨酶,并分为转氨酶正常组和转氨酶增高组各25例,另选择10例健康志愿者为正常对照组,对上述各组采用常规外周血淋巴细胞染色体制作,G显带进行常规染色体核型分析,并采用同步银染法观察染色体着丝粒点变异情况,分析变异率与乙型肝炎病毒感染的关系。结果转氨酶正常的乙型肝炎表面抗原阳性者的外周血淋巴细胞染色体着丝粒点变异率高于正常对照组,但无显著性差异,而转氨酶增高者则显著高于两组,之间具有显著差异。结论乙型肝炎病毒可引起外周血染色体着丝粒点变异,而导致体细胞有丝分裂和生殖细胞减数分裂时染色体复制错误或丢失,是引起孕妇于孕早期胚胎异常的原因之一。Objective To study the relationship of the aberration of peripheral blood chromosomal centrimeric dots （Cd） and the infection of hepatitis B for the women with spontaneous abortion. Methods Fifty cases of women who have history of spontaneous abortion were studied. We examined hepatitis B antigen in their blood with ELLASA. Those women with hepatitis B antigen were examined further aminotransferase and were divided into a group of normal aminotransferase and a group of abnormal aminotransferase according to the results. A control group was 10 healthy women. Three groups above were examined chromosome. Kayrotpes were analyzed by G banding and centromeric dots were ana- lyzed by Simultaneous silver attaining technique. Results There was no different significantly between the group of normal aminotransferase and the group of healthy women in the rate of aberration of Cd. The rate of the group of abnor- mal amniotransferase was higher than the two group above and there was different significantly. Conclusions Hepatitis B can lead to change to Cd. And the aberration of Cd cause erro in the during of meiosis of germ cells or delete which can lead to aneuploid aberration.

关 键 词：自然流产 外周血 染色体 染色体着丝粒点 乙肝病毒 

分 类 号：R512.62[医药卫生—内科学]