霉酚酸酯对人肝癌细胞HepG-2抑制作用的研究  

Inhibitory effect of mycophenolate mofetil on Human Hepatoma cell HepG-2

作　　者：刘屹[1] 褚延魁[1] 王成果[1] 臧莉[1] 杨媛[1] 杜锡林[1] 董瑞[1] 宋舟[1] 江雪[1] 鲁建国[1] 马庆久[1] 

机构地区：[1]第四军医大学唐都医院普外科,陕西西安710032

出　　处：《现代生物医学进展》2008年第3期478-480,共3页Progress in Modern Biomedicine

基　　金：陕西省自然科学基金(2005K09-G12-4)

摘　　要：目的:研究霉酚酸酯体外对细胞生长抑制率、细胞凋亡以及对细胞黏附率的影响。方法:以霉酚酸酯在0.1μg/ml-100μg/ml,24-72h内作用于肝癌细胞,MTT法检测肿瘤细胞的生长抑制率,流式细胞仪检测细胞周期,Hoechst33258荧光染色观察细胞凋亡的形态变化,细胞黏附实验检测细胞黏附率的影响。结果:霉酚酸酯显著的抑制了肿瘤细胞的增长,并显著的抑制其黏附率,在浓度为100μg/ml作用72小时时生长抑制率达78.8%,黏附率降低至42.1%,Hoechst33258染色实验发现随浓度增大细胞凋亡的发生增多,核固缩、核碎裂的现象发生越明显。流式细胞仪检测,细胞周期阻滞于GO/G1期,减少增殖细胞在S期的分布。结论:霉酚酸酯对肝癌细胞HepG-2的增长具有明显的抑制作用。Objective： To investigate the effects of mycophenolate mofefil on cell growth, cell adhesion and apoptosis of human hepatoma cell HepG-2 in vitro. Methods： Mycophenolate mofetil（ 0.1 μg/ml-100μg/ml） were added into the cultured HepG-2 cells in vitro for 24-72 h. The inhibition ratio of hepatoma cells was examined by MTT assay; cell cycle was tested with flow cytometry; cell apoptosis was observed by using Hoechst33258 staining; and cell adhesion were detected by adhesion test. Results： Mycophenolate mofetil inhibited the growth and adhesion rate of HepG-2 cells significantly .When its concentration was 100μg/ml,acting for 72 h, inhibition rate of HepG-2 cell growth reached to 78.8%, and the adhesion rate lowered to 42.1%. By Hoechst 33258 staining, it was discovered that apoptosis occurred increasingly with enlargement of the concentration. By flow cytometry, the cell cycle was blocked at G0/GI phase, decreasing the distribution of proliferation cells in S phase. Conclusion： Mycophenolate mofetil can obviously inhibit the growth of HepG-2 cells.

关 键 词：霉酚酸酯 肝癌 凋亡 细胞周期 黏附性 

分 类 号：R285.6[医药卫生—中药学]